Based on our advanced Chemokine Production Measurement Service and powerful technology, Creative Biolabs has developed an unparalleled platform for assays of production measurement of CXCL, to meet our customers' different purposes. Our highly skilled scientists are ready to get started on your projects.

Introduction of chemokine CXCL

Chemokines, chemotaxis mediators for the directional migration of effector cells, play a vital role in the angiogenesis and tumorigenesis in immune responses. Chemokines characterized by four highly conserved cysteine amino acid residues are classified into four subfamilies: CC, CXC, CX3C, and XC. The chemokine ligand CXCL (C-X-C motif in the N-terminal) subfamily is one main component of the four distinct chemokine groups.

• Functions of chemokine CXCL

The chemokine CXCL subfamily has about 17 component chemotactic cytokines. They play an essential role in inflammation and cancer development. Some chemokine CXCL could direct immune cells with antitumor effects. As shown in Fig.1 (left), CXCR3 and its ligands CXC-chemokine ligand 9 (CXCL9), CXCL10, and CXCL11 together play a key role in the recruitment of TH1 cells, CD8+ T cells, and NK cells into the tumor microenvironment, which results in the decrease of cancer metastasis and helps achieve the tumor regression.

While chemokines, expressed in the tumor microenvironment by tumor cells or tumor associate cells like myeloid cells, could recruit pro-tumor immune cells and inhibit antitumor immune responses. As shown in Fig.1 (right), CXCR1/2 with its ligand CXCL8, CXCR2 with its ligand CXCL5, and CXCR4 with its ligand CXCL12 together promote and maintain cancer stemness and angiogenesis, leading to cancer progression. Therefore, the detection of chemokine CXCL is essential for cancer immunotherapies, including the Engineering Leukemia Cell with the self-destruction strategy.

Fig.1 The immunity promotion and pro-tumor effects of chemokines. (Nagarsheth, et al., 2017)

Chemokine CXCL Production Measurement Services at Creative Biolabs

The assessment of the CXCL expression level in samples may represent the systemic circulation and tumor microenvironment. Detection of the CXCR expression in tumor tissues and measurement of the tumor-infiltrating immune cell subsets are of great value for cancer immunotherapy research.

To determine the production of CXCL, we will use classical routine immunological methods, such as the Enzyme-linked Immunosorbent Assay (ELISA) and Western Blot (WB) assay. While they may have some disadvantages like hours of assay time and low sensitivity, new biosensors with electrochemical and fluorescent techniques such as surface plasmon resonance (SPR), chemiluminescence, and electrochemical impedance spectroscopy (EIS) have appeared with a growing trend. These rising methods have shown inherited ideal analytical merits including real-time detection, high sensitivity, and convenient automatization. Creative Biolabs provides CXCL measurement services suitable for our customers.

With industry-leading expertise and state-of-the-art single-use equipment, Creative Biolabs has pioneered the measurement of chemokine CXCL production and their receptors' expression. These parameters will be measured at Creative Biolabs with various methods including flow cytometry (FC), enzyme-linked immunoabsorbent assay (ELISA), and Real-time PCR, as described in the related page Chemokine CCL.

Features of CXCL Production Measurement Service

✔ Advanced technical platform with high sensitivity, accuracy, and repeatability

✔ Fully customizable design to meet every specific demand

✔ Fast turnaround time

Based on advanced facilities, capabilities, and expertise in chemokine science, Creative Biolabs is confident in solving the problems associated with chemokine. Our comprehensive services include chemokine detection and related cancer immunotherapy projects. If you are interested in our services, please feel free to contact us directly.

Reference

1. Nagarsheth, N.; et al. Chemokines in the cancer microenvironment and their relevance in cancer immunotherapy. Nature Reviews Immunology. 2017,17(9): 559-572.

For Research Use Only | Not For Clinical Use