Creative Biolabs has established and continues to scale, a powerful platform to engineer cancer cells with therapeutic gene circuits for cancer immunotherapy. Based on our platform, we have experience in engineering the HCT116 cell line, thus providing a promise for the next cancer therapeutic breakthrough.

Introduction of HCT116 Cell Line

The HCT116 cell line is known to be a highly aggressive cell line with little or no capacity to differentiate. It was isolated from the epithelial tumorigenic colon tissue of an adult male patient with colorectal carcinoma. The cells are adherent with an epithelial morphology and have a mutation in codon 13 of the KRAS proto-oncogene. The HCT116 cell line, which has a pseudo-diploid karyotype, is a popular model in the fields of cancer cell biology, intestinal immunity, and inflammation. HCT116 cells show high metastatic potential. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases (lung, liver, and lymph node metastases). Creative Biolabs has established a platform to turn cancer cells against themselves. Based on this platform, we've cultivated a talented team of experts who have rich experience in engineering the HCT116 line.

Engineering HCT116 Cell Line Service

The design of the highly complex tumor-killing gene circuits was made by meticulous optimization and integration of several components. Circuit-mediated immunomodulation mediates effective anti-tumor responses by activation of immune cells and regulation of cancer signaling networks. Scientists at Creative Biolabs help our clients design and construct gene circuits for the development of smarter strategies to address cancer challenges.

• Strategies for Engineering HCT116 Cell

Engineered cancer cells can be a useful tool for clinical and therapeutic applications in cancer treatment. Based on our platform, we provide several off-the-shelf strategies to engineer HCT116 cells for cancer self-destruction. The strategies include engineering cancer cells with various cytokines, aptamer, aptamer-siRNA chimera, etc.

• Technologies of Engineering HCT116 Cell

Our scientists have extensive expertise and experience in gene delivery and cell line engineering, which enables the construction of a variety of cell lines through lentivirus infection, retrovirus, CRISPR technology, etc. thus shortening your project turnaround time.

• Additional Assay Services

Creative Biolabs is one of the few providers of integrated upstream generation & downstream assessment services. We offer additional assay services after the completion of your custom cell project. Our comprehensive assays and assay development services cover all major therapeutic target classes including but not limited to:

Creative Biolabs is pleased to offer custom cancer cell engineering services addressing multiple components of the cell engineering workflow. Our state-of-the-art labs are staffed by some of the best-trained and most experienced subject matter experts in the field. We custom-design the optimal engineering strategy for each project to meet your requirements. If you are interested in our services, please feel free to contact us to discuss your project.

Reference

1. Nagai, E.; et al. Irradiated tumor cells adenovirally engineered to secrete granulocyte/macrophage-colony-stimulating factor establish antitumor immunity and eliminate pre-existing tumors in syngeneic mice. Cancer Immunology, Immunotherapy. 1998, 47(2): 72-80.

For Research Use Only | Not For Clinical Use