Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that allows the localization of a specific DNA sequence or an entire chromosome in a cell using fluorescently labeled probes. The FISH analysis appears to be an excellent tool for molecular profiling of various cancers at the single-cell level due to its specificity, simplicity, and reliability. Creative Biolabs offers a full line of FISH services through a comprehensive set of methods, state-of-the-art equipment, and continuous development.

Fig.1 FISH technology. (Creative Biolabs)

FISH-based Tumor Profiling Services

Tumor profiling has become fundamental not only informing on tumor diagnosis and prognosis but also providing important information for decision-making in the treatment of cancer patients. Currently, FISH is widely used for detecting specific genomic aberrations and providing important information for cancer diagnosis, risk stratification, and prognosis. As your lab partner, Creative Biolabs offers full FISH service solutions tailored to your project needs from probe design to result interpretation. Our laboratory professionals with a wide range of experience and education will work with you to make sure the specifics of the testing meet your needs. Our experts provide assistance and support in the design and selection of FISH probes in order to deliver consistent, reliable results for your project.

Our FISH Capabilities

FISH assays play a critical role in establishing a diagnosis, prognosis, and prediction of response to therapy, and provide the basis of precision medicine. Creative Biolabs offers various FISH services to meet your characterization needs.

Key Features & Advantages

• Personalized experimental schemes could be customized according to customers' needs
• High-quality, timely results
• Cost-effective testing strategies

FISH Workflow

FISH has evolved to become a vital diagnostic tool for personalized medicine. Creative Biolabs provides customized FISH-based tumor profiling services to reveal a lot about a tumor. Our lab utilizes state-of-the-art equipment to provide accurate results with a rapid turnaround time. Our standard workflow shows as follows.

One-stop Tumor Profiling Platform

Various technologies have been used for tumor profiling including Next-generation Sequencing (NGS), polymerase chain reaction, mass spectrometry, in-situ hybridization, microarrays, and others. Creative Biolabs works with you to choose ideal solutions to ensure the best tumor profiling results. For complex tumor profiling projects, our scientists use a combination of technologies to uncover cancer information. For more details, please contact us and we will discuss your needs and what we can offer.

Published Data

Data 1: Frequent molecular abnormalities were detected by FISH in pediatric glioblastomas.

Sample type: formalin-fixed, paraffin-embedded, 5-μm tissue slides

Tumor: glioblastomas

Technology: Dual-color FISH

Fig.2 FISH analysis of pediatric glioblastomas. (Korshunov, et al., 2005)

Data 2: FISH is a diagnostic aid in ambiguous melanocytic tumors

Sample type: formalin-fixed, paraffin-embedded, 5-μm tissue slides

Tumor: melanocytic tumor

Technology: Multiple-color FISH

Fig.3 Example of FISH-positive ambiguous lesion. (Vergier, et al., 2011)

References

1. Korshunov, A.; et al. Clinical utility of fluorescence in situ hybridization (FISH) in nonbrainstem glioblastomas of childhood. Modern pathology. 2005, 18(9): 1258-63.
2. Vergier, B.; et al. Fluorescence in situ hybridization, a diagnostic aid in ambiguous melanocytic tumors: European study of 113 cases. Modern Pathology. 2011, 24(5): 613-23.

For Research Use Only | Not For Clinical Use