HPLC-MS/MS-based Half-life Assessment Service
High-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) is a common method for assessing the half-lives of drugs and their metabolites. Creative Biolabs provides a wide range of drug development and analytical testing services, including HPLC-MS/MS-based half-life assessment service of drugs and metabolites.
Why Choose Creative Biolabs for Half-life Assessment
Experience & Expertise: Creative Biolabs has extensive experience and talented scientists in HPLC-MS/MS analysis and pharmacokinetic studies.
Technology & instrument: Creative Biolabs has state-of-the-art HPLC-MS/MS equipment and infrastructure to deliver high-quality results.
Turnaround time & capacity: Creative Biolabs has the ability to handle your sample volume and meet your project timeline.
HPLC-MS/MS-based Half-life Assessment Service at Creative Biolabs
Creative Biolabs offers comprehensive services and adheres to high standards, making our excellent choices for your pharmacokinetic testing needs. Creative Biolabs uses a high-throughput HPLC-MS/MS-based assay to quantify the percentage of compounds remaining after incubation to determine metabolic stability in plasma and blood from different species. Finally, the half-life (T1/2) is estimated from the slope (%) of the initial linear range of the remaining logarithmic curve of the compound as a function of time.
Sample Preparation
Biological samples containing drugs or compounds, such as blood, plasma, or tissue, are prepared for analysis. This step involves dilution, filtration, derivatization, etc., to make the sample suitable for HPLC.
Chromatographic Separation
The sample prepared in the previous step is injected into the HPLC system.
Detection and Quantification
The separated components are introduced into the mass spectrometer.
Data Analysis
Concentrations of drugs or compounds are measured at different time points. Half-life results are calculated by plotting relevant parameter relationships and fitting data to an appropriate pharmacokinetic model.
Detailed Information of Our Service
Creative Biolabs has extensive experience and an in-depth understanding of pharmacokinetic studies and instrument configurations, which can help you achieve accurate quantification of low-abundance compounds efficiently and quickly. This method can be used for the analysis of a wide range of compounds, including small molecules, peptides, and proteins.
Research Area of HPLC-MS/MS-based Half-life Assessment
HPLC-MS/MS-based half-life assessment is a critical tool in various scientific fields. It provides precise and reliable data that are essential for drug development, therapeutic drug monitoring, and safety assessments. By understanding the half-life of compounds, it is possible to improve the effectiveness, safety, and overall success of drugs and therapeutic interventions.
You can click the keywords to get more information about our In Vivo Drug Metabolism and Pharmacokinetics (DMPK) Services:
Frequently Asked Question
A1: What is a half-life assessment?
Q1: Half-life assessment is a critical component of pharmacokinetic studies, used to determine the time it takes for the concentration of a drug or other substance in the body to be reduced by half.
Half-Life (T1/2):
The half-life of a substance is the time required for its concentration in the blood or plasma to decrease by 50%. It is a measure of the rate at which a drug is eliminated from the body.
Contact Us
Whether you have questions about our HPLC-MS/MS-based half-life assessment service or want to discuss how we can meet your specific requirements, our team is ready to assist you. Please feel free to contact us in any way you want. Our customer service representatives are available to provide you with the support you need. We look forward to hearing from you!
For Research Use Only | Not For Clinical Use
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