In Vivo PK Service
In Vivo PK
"In Vivo PK" stands for "In Vivo Pharmacokinetics." Pharmacokinetics (PK) is the study of how a drug moves through the body, including absorption, distribution, metabolism, and excretion (ADME). "In Vivo" refers to studies conducted in living organisms, typically animals or humans, rather than in vitro (in a controlled laboratory environment). In Vivo PK studies are crucial for understanding how a drug behaves in a biological system, which is essential for determining proper dosing regimens, predicting potential side effects, and assessing overall drug efficacy.
Fig.1 Classification of animal models.1
In preclinical studies, important in vivo PK parameters for compounds include:
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Clearance (CL)
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Apparent volume of distribution (Vd)
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Half-life (t1/2)
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Bioavailability (F%)
Our Service
Creative Biolabs has many years of experience in animal in vivo PK experiments, providing reliable, flexible and cost-effective services to customers around the world. Creative Biolabs offers a variety of in vivo PK assay services, including but not limited to:
Why Choose Creative Biolabs
Based on a complete technology platform and experienced scientists, Creative Biolabs has established a complete testing service process in the field of in vitro ADME detection of antibodies and proteins, which can provide you with a one-stop solution to meet your analysis needs.
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One-stop ADME service to provide a comprehensive solution for evaluating drug metabolism and safety characteristics
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Cost-effective, high-quality, reproducible data, and fast turnaround time
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Flexible, customized solutions to meet your specific testing needs
Frequently Asked Questions
Q1: In the in vivo PK test, why is CL greater than Qh?
A1: (1) The liver is the most important organ for drug metabolism, but it is not the only way to eliminate drugs, as well as kidney elimination; (2) The drug is unstable in the blood, and the clearance of the drug is not a liver effect; (3) In some cases, when the drug concentration in red blood cells is much higher than the drug concentration in plasma, the calculation of the true clearance rate cannot be used to approximate the drug concentration in the blood by the drug concentration in the plasma; (4) In individual cases, the metabolism or uptake of the lungs occupies a major role.
Q2: Why is the bioavailability greater than 100% in the in vivo PK test?
A2: (1) Dose deviation caused by dosing error; (2) nonlinear PK; (3) High bioavailability and individual differences in the DMPK attributes of the drug, IV and PO were given to different groups of animals; (4) unreasonable sampling; (5) the IV sample was left out for a longer period and the compound was unstable in plasma; (6) When administered in the form of racemates, the enantiomers with faster clearance are converted into enantiomers with slower clearance in the digestive tract.
Q3: What should I do if there is a large difference in AUC in the in vivo PK test?
A3: (1) The reason for the large difference can be summarized as the poor solubility of the compound, or the solubility fluctuates greatly by the pH value of the digestive tract; (2) Improve the preparation and adjust the pH value of the digestive tract.
To learn more about our DMPK services, click here or contact us now and one of our scientists will get back to you as soon as possible.
Reference
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Domínguez-Oliva, Adriana et al. "The importance of animal models in biomedical research: current insights and applications." Animals : an open access journal from MDPI. (2023) 13,7 1223.
For Research Use Only | Not For Clinical Use
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