Immuno-oncology Phospho-flow Cytometry Service

Unveiling Intracellular Signaling in Cancer: Precision Beyond Surface Markers

Creative Biolabs proudly presents our Immuno-oncology phospho-flow cytometry service, a cutting-edge solution designed to delve into the intricate world of intracellular signaling in cancer cells. By combining the power of flow cytometry with phospho-specific antibodies, we provide unparalleled insights into the phosphorylation status of key signaling molecules, unlocking new avenues for targeted cancer therapies.

Specific Services

• Phospho-specific Antibody Selection
  Our experts assist in the selection of phospho-specific antibodies targeting critical signaling molecules implicated in cancer pathways, ensuring comprehensive coverage of relevant phosphorylation events.
• Optimized Staining Protocols
  We develop and optimize staining protocols tailored to the unique characteristics of each sample type, including cell lines, patient-derived xenografts (PDX), and primary tumor samples, to achieve maximum staining efficiency and signal-to-noise ratio.
• Multiplex Phospho-flow Panel Design
  Leveraging our extensive expertise, we design multiplex phospho-flow panels with precise combinations of antibodies targeting phosphorylated and non-phosphorylated epitopes, allowing simultaneous assessment of multiple signaling pathways within individual cancer cells.
• Advanced Flow Cytometry Analysis
  Our state-of-the-art flow cytometry platforms enable high-dimensional analysis of intracellular signaling events, including phospho-specific staining combined with surface marker expression profiling, cell cycle analysis, and apoptosis assays, to provide a comprehensive understanding of cancer cell biology.
• Data Standardization and Interpretation
  We standardize data acquisition and analysis parameters across experiments to ensure reproducibility and comparability of results. Our experts provide detailed interpretation of phosphorylation patterns, pathway activation profiling, and statistical analysis to extract meaningful insights from complex phospho-flow cytometry data.
• Customized Assay Development
  We also offer customized assay development services to meet specific research needs, including the optimization of staining conditions, validation of assay performance using control samples, and adaptation of protocols to unique experimental requirements or sample limitations.

By offering these comprehensive and customizable services, we empower researchers to gain deep insights into intracellular signaling dynamics in cancer cells, paving the way for the development of targeted therapies and precision medicine approaches.

Sample Requirements

• High-quality single-cell suspensions from cancer cell lines, patient-derived xenografts, or primary tumor samples.
• Adequate sample size and viability to ensure robust staining and data acquisition.
• Detailed information on sample treatment conditions and prior exposure to therapeutic agents, if applicable.

Key Points

• Rigorous optimization of staining protocols to minimize non-specific binding and maximize signal-to-noise ratio.
• Validation of assay specificity and sensitivity using positive and negative control samples.
• Standardization of data acquisition and analysis parameters to ensure reproducibility and comparability across experiments.

Fig.1 Phospho-specific flow cytometry reveals aberrant signaling in indolent B-cell lymphoma.¹

Advantages

• Provide detailed insights into the phosphorylation status of key signaling molecules within cancer cells.
• Tailor assay protocols and panel designs to specific research questions or experimental requirements.
• Achieve sensitive detection of phosphorylation events with low background noise, enabling accurate quantification of signal intensity.
• Offer comprehensive data analysis and interpretation to facilitate the translation of experimental findings into actionable insights.

In summary, our immuno-oncology phospho-flow cytometry service offers a comprehensive suite of advanced techniques and expertise to delve into the intricate signaling pathways of cancer cells. With tailored assay development, multiplex panel design, and in-depth data analysis, we provide researchers with valuable insights into aberrant signaling dynamics in cancer, facilitating the discovery of novel therapeutic targets and the development of precision cancer therapies. Contact us today to explore how our phospho-flow cytometry service can accelerate your research and propel advancements in cancer treatment.

Reference

1. Blix, Egil S., et al. "Phospho-specific flow cytometry identifies aberrant signaling in indolent B-cell lymphoma." BMC cancer 12 (2012): 1-12.

For Research Use Only | Not For Clinical Use

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