Highly Accurate Subpopulation Analysis by Flow Cytometry

Creative Biolabs provides cutting-edge flow cytometry assays to enable immunophenotyping and qualification of markers on immune cells. We offer a one-stop solution from consultation about the experiment design, preparation of cells (tissue dissociation, cell isolation, staining, and acquisition), to result analysis.

Our analysis instruments have up to 21 colors, which have been widely applied in research settings. This technology can be used in cell counting, cell sorting, identification of various cell populations, and biomarker detection. Moreover, a wide range of cell types are available, including bacteria, yeast, and mammalian cells. Our result supports a deep interrogation of the immune response elicited from novel therapeutics.

Achieve a Comprehensive Analysis of the Immune System

Flow Cytometry Assay Development Services

Although flow cytometry has excellent performance in detecting rare cell populations and quantifying cellular phenotypes in biological samples, methods of flow cytometric are challenging to develop and validate, especially given the complexity of the samples, the lack of standardized cellular references, and the complexity of measurements involved. However, when designed appropriately, it will provide a wealth of information. Creative Biolabs offers one-stop flow cytometry assay development services, which ensure the whole process can be designed and optimized accordingly.

Fig.1 The process of flow cytometry assay development.

Advantages of Our Flow Cytometry Assay Service

• Multicolor
• Accurate and effective
• Incredibly rich data sets, bringing a ray on biological events at single-cell resolution.
• Can be used in immune monitoring
• Can be used in the analysis of natural killer (NK) cell cytotoxicity

Publications Sharing Based on Flow Cytometry Assays

Publication 1

Cells Used: Human epidermoid cancer cell lines (KB and KB_V200 cells)

Journal: Methods in molecular biology

IF: 0.368

Research Findings: Flow cytometry is a highly effective technology to detect intracellular cytokines using specific fluorescence-labeled antibodies. This paper described a modified method to detect the intracellular cytokines using flow cytometry. It emphasized the effects of variables including samples, buffers, temperature, data achievement, and result analysis.

Fig.2 The expression of P-gp/IL-8 in KB and KB_V200 cells was analyzed by flow cytometry.¹

Publication 2

Cells Used: Human epidermoid cancer cell lines (KB and KB_V200 cells)

Journal: Cytometry B Clin Cytom

IF: 3.4

Research Findings: The paper assessed the feasibility of the flow cytometry technique to follow CAR T-cells in an infusion bag and patient's blood. The researchers assessed CAR T-cell heterogeneity in terms of CD4⁺ and CD8⁺ T lymphocytes within the subset. Moreover, this technique allows monitoring of both authority-approved CD19 CAR T cells.

Fig.3 Gating strategy for antiCD19 CAR T-cell from infusion bag.²

For more details about our immuno-oncology flow cytometry assay service, please feel free to contact us.

References

1. Qiu, Jian-Ge, et al. "Cytokine detection by flow cytometry." Cytokine Bioassays: Methods and Protocols (2014): 235-242.
2. Demaret, Julie, et al. "Monitoring CAR T‐cells using flow cytometry." Cytometry Part B: Clinical Cytometry 100.2 (2021): 218-224.

For Research Use Only | Not For Clinical Use

Related Services

• Immuno-oncology Immunophenotyping Service
• Immuno-oncology Receptor Occupancy (RO) Measurement Service
• Immuno-oncology Phospho-flow Cytometry Service