Empowering Precision in Immunotherapy through RO Insights

Creative Biolabs offers an advanced Immuno-oncology receptor occupancy (RO) measurement service tailored to dissect these crucial interactions. By quantifying receptor engagement, we unveil critical insights into the efficacy and mechanisms of action of immunotherapeutic agents, facilitating the optimization of therapeutic strategies for combating cancer.

Specific Services

• Receptor Occupancy Assays

Leveraging cutting-edge techniques like Flow Cytometry-based or ligand-binding assays, we quantitatively measure the occupancy of immune receptors by therapeutic agents.

• Dose-response Analysis

Our service encompasses dose-response studies to evaluate receptor occupancy across a spectrum of drug concentrations, enabling the determination of optimal dosing regimens.

• Customized Assay Development

We specialize in tailoring assay protocols to specific receptors and therapeutic agents, ensuring precise and reliable measurements.

• Comprehensive Data Analysis

Our analysis includes comprehensive data interpretation, encompassing dose-response curves, EC50 determination, and robust statistical analysis to facilitate informed decision-making.

Sample Requirements

• High-quality purified antibodies or ligands.
• Relevant cell lines or primary cells expressing the target receptor.
• Detailed information on the therapeutic agent, including concentration, formulation, and stability.

Advantages:

• Quantitative Precision: Offer precise measurements of receptor occupancy, enabling quantitative evaluation of drug-target interactions.
• Customization: Tailor assay protocols to specific receptors and therapeutic agents, ensuring relevance and accuracy.
• Comprehensive Insights: Provide in-depth data analysis and interpretation, empowering informed decision-making in drug development and clinical research.
• Expertise and Support: Benefit from our team's expertise and state-of-the-art facilities, delivering reliable and high-quality results.

Publications Sharing

Assay type: RO Profiling

Journal: MAbs

IF: 5.3

Research Findings: RO measurements from various assays may exhibit significant discrepancies when the internalization rate of bound receptors surpasses the degradation rate of free receptors.

Fig.1 Observed and model predicted profiles of PD-1 occupancy.¹

FAQs

1. What is Immuno-oncology RO Measurement?

RO Measurement is a specialized service used to quantify the engagement of therapeutic antibodies or ligands with immune receptors in the context of cancer immunotherapy.

2. Why is measuring receptor occupancy important in cancer immunotherapy?

Measuring receptor occupancy provides crucial insights into the pharmacokinetics, pharmacodynamics, and efficacy of immunotherapeutic agents. It helps optimize dosing regimens, assess treatment response, and understand the mechanisms of action underlying therapeutic effects.

3. What techniques are used for RO measurement?

RO measurement can be performed using various techniques, including flow cytometry-based assays, ligand-binding assays, and imaging-based methods. These techniques enable quantitative assessment of receptor occupancy with high sensitivity and specificity.

4. What types of samples can be analyzed using this service?

This service can analyze a wide range of samples, including cell lines, primary cells, patient-derived samples (e.g., blood, tissue biopsies), and preclinical or clinical trial samples.

Our immuno-oncology RO measurement service stands ready to provide you with precise insights to optimize your therapeutic strategies. Contact us today to leverage our RO Measurement service. Let's collaborate to elevate your research and drive innovation in targeted cancer solutions.

Reference

1. Shchelokov, Dmitry, and Oleg Demin Jr. "Receptor occupancy assessment and interpretation in terms of quantitative systems pharmacology: nivolumab case study." MAbs. Vol. 15. No. 1. Taylor & Francis, 2023.

For Research Use Only | Not For Clinical Use