Reaction Phenotyping Assessment Service
Reaction Phenotyping Assessment in Drug-Drug Interactions
Reaction phenotyping assays are valuable tools in drug development and pharmacology research. These assays aim to evaluate the role of specific enzymes or transporters in the metabolism of a drug. These assays are used to identify and quantify the enzymes responsible for drug metabolism, which can help predict how a drug will be metabolized in the body and its potential interactions with other drugs. The information obtained from reaction phenotyping assays can be crucial in drug development and personalized medicine, as it can contribute to optimizing drug dosages, improving drug efficacy, and minimizing the risk of adverse drug reactions.
Reaction Phenotyping Assay
At Creative Biolabs, we offer reaction phenotyping assessment services to identify the specific enzymes involved in the metabolism of a drug, predict potential drug-drug interactions, and assess the overall metabolic clearance of the drug in humans. Our reaction phenotyping assays involve incubating the drug of interest with recombinant enzymes and/or human liver microsomes. By monitoring the formation of metabolites, we are able to determine which enzymes are responsible for the metabolism of the drug.
Recombinant enzyme system: Recombinant enzymes are expressed in cell systems and used to study the metabolism of drugs. By incubating the specific enzyme with the drug, researchers can determine the enzyme's specific role in drug metabolism.
Human liver microsome system: Liver microsomes contain the enzymes responsible for drug metabolism. By incubating the microsomes with the drug in the presence of specific enzyme inhibitors and analyzing the metabolites formed, researchers can determine which enzymes are responsible for metabolism.
Creative Biolabs offers CYP and UGT reaction phenotyping assays tailored to the client's specific requirements.
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CYP isoforms: CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A
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UGT isoforms: UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9, UGT2B7, and UGT2B15
For other isoforms please inquire us.
Parent depletion and metabolite formation assays are two common approaches for reaction phenotyping.
Protocol
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Test Article Concentration
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5 μM (custom concentrations available)
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Test System
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Recombinant enzymes or human liver microsomes
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Enzymes
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CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6,CYP3A, UGT1A1, UGT1A4, UGT1A6, UGT1A9, UGT2B, etc.
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Time Points
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0, 5, 15, 30, 45 minutes (custom time points available)
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Number of Replicates
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3
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Analysis Method
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LC-MS/MS
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Readout
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Parent depletion or specific metabolite formation
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About Our Service
Our services are designed to provide comprehensive data on drug metabolism, enabling you to make informed decisions about drug development and dosing. Our reaction phenotyping assays are highly sensitive and specific, ensuring accurate results. Additionally, our team of experienced scientists is dedicated to providing you with prompt and reliable support throughout the entire process. Whether you need assistance with assay design, data interpretation, or any other aspect of reaction phenotyping, we are here to help. Contact us today to learn more about our reaction phenotyping assessment services.
Reference
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Harper, et al.; "Reaction phenotyping: current industry efforts to identify enzymes responsible for metabolizing drug candidates." The AAPS journal 10 (2008): 200-207.
For Research Use Only | Not For Clinical Use
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