Creative Biolabs is a CRO company specializing in the emerging field of tumor lineage tracing. Building on the unmatched expertise in single-cell sequencing, our mission is to develop and provide new single-cell transcriptome sequencing lineage tracing methods to research communities around the world. Our lineage traceing system can analyze the gene expression of thousands of tumor single cells, with pre-designed or customized analysis methods that are flexible enough to meet any needs of our worldwide customers.

Introduction to Single-cell Transcriptome Sequencing

Single-cell Transcriptome Sequencing, also known as single-cell RNA-sequencing (scRNA-seq), has proven its significant role in tumor heterogeneity and tumor evolution research. it quantifies the transcriptome of individual cells, especially for tumor cells. As a result, Creative Biolabs introduces a wide range of industry-recognized single-cell analysis systems that rapidly parallel analyze the expression levels of various genes in thousands of single cells.

In our BD Rhapsody platform, we utilize a microporous-based method to isolate and label single cells, employing a microporous plate with no less than 100,000 specific-sized microholes capable of capturing and loading magnetic beads onto each cell.

In our 10X genomics platform, we can distinguish different cells and transcripts in the population by adding specific sequence tags, and obtain the digital gene expression profile at the single-cell level, which can realize the analysis of at least thousands of single-cell populations, and solve the shortcomings of conventional scRNA-seq methods in terms of throughput or scalability, and open up new insights for single-cell research.

Benefits

• High-quality nuclear extraction: rich experience in nuclear extraction, optimized solutions for different organizations or cells
• Standardized internal control: Rich practical experience in building a standardized internal control system
• Process analysis: well-mature analysis process, accurate and rapid analysis of single nucleus transcriptome data
• Professional team: professional technical team with many years of project design, experimental operation, after-sales analysis and other experience
• One-stop solution: provide a full range of services for tissue extraction, single nucleus capture, reverse transcription, library sequencing, and data analysis

Fig.1 Development of Single-Cell RNA Sequencing Technology.¹

Sample Requirement

1. > 10,000 cells;
2. Concentration: > 500-1,000 PCS /uL;
3. No adhesion between cells;
4. No cell debris or other particles larger than 30um;
5. The survival rate of cells should be greater than 85%;
6. Tissue RNA does not degrade, RIN≥7;
7. No reverse transcription inhibitors and non-cellular nucleic acid molecules;
8. Sample preparation: tissue frozen with liquid nitrogen and stored at -80°C;
9. Mode of transport: dry ice transport.

Single-cell Transcriptome Sequencing Lineage Tracing Services

Over the past decade, Creative Biolabs has focused on generalizing and inferring the universal formulas that determine the fate of different tumor cells for accurate and customized cancer diagnosis research. Therefore, we have developed a novel single-cell transcriptome sequencing lineage tracing technique that can simultaneously measure cell lineage and cell state at the same cellular level. Up to now, we can provide both prospective lineage tracing and retrospective lineage tracing strategies for measuring the state of different dimensions of cells.

In a CRISPR-Cas9-mediated scRNA‐seq project, first, we can distinguish each tumor cell by introducing CRISPR-Cas9 DNA endonuclease and sgRNA-specific markers. we then add these lineage barcodes to the transcriptional regions that control the expressed or induced reporter genes, making their sequences readable from the mRNA level, so that both transcriptome and cell fate information can be obtained when performing single-cell transcriptome sequencing.

Fig. 2 Methods and Logic for Lineage Barcoding Experiments.²

Advantages

• Specializing in single-cell genomics and transcriptomics
• End-to-end solution from isolated single cells to lineage tracing
• High-quality, innovative single-cell and spatial multi-omics services
• High-throughput single-cell gene expression profiling solutions
• Authoritative certification reports

Creative Biolabs opens a whole new field in lineage tracing based on single-cell transcriptome sequencing to allow the real-time study of tumor cell fates. Since 2006, we have accumulated unrivaled expertise in single-cell genomics and transcriptomics, and our scientists have extensive experience in single-cell RNA sequencing and data analysis. We look forward to collaborating with you and providing high-quality human oncology research services. If you have any further questions, please feel free to contact us, and we will promptly respond to your inquiries via messages or phone calls.

References

1. Jovic, D.; et al. Single-cell RNA sequencing technologies and applications: A brief overview. Clinical and Translational Medicine, 2022, 12(3): e694.
2. Wagner, D. E.; et al. Lineage tracing meets single-cell omics: opportunities and challenges. Nature Reviews Genetics, 2020, 21(7): 410-427.

For Research Use Only | Not For Clinical Use