Creative Biolabs provides a one-stop solution for oncology imaging services. We offer high-quality, affordable lineage tracing services, with world-class support from our highly experienced single-cell sequencing project experts. We can help you stay ahead of the curve. We are committed to continuously integrating cutting-edge tumor single-cell lineage tracing technologies into our service offerings to ensure that customers have access to the latest advancements. Welcome to inquire!

Introduction to Tumor Single Cell Sequencing

Heterogeneity plays an important role in the study of tumors. In general, tumor heterogeneity can be analyzed by sequencing technology, but conventional sequencing technology can only explain the differences among tumor samples, and its resolution is too low and cannot reach the level of single cells. Therefore, Creative Biolabs is keen to obtain data on tumor cell type, tumor cell state and subpopulation distribution, tumor cell-cell interactions, cell origin, and fate at the single-cell level.

In our labs, the single-cell sequencing procedure consists of several parts, single-cell isolation, nucleic acid amplification, library construction, high throughput sequencing, and data analysis. The key to successful single-cell sequencing is to extract and obtain complete and active target cells. To date, we offer a variety of single-cell separation platforms, including but not limited to, fluorescent cell sorting, immunomagnetic separation, as well as microfluidic platforms. Our high-throughput platform has been recognized by a large number of customers due to its low cost, ease of operation, high degree of process automation, and high volume of data analysis. Besides, in the process of dynamic development or differentiation of tumor cells, our trajectory analysis assays can be used to obtain the direction and state of cell development. It also helps to obtain intermediate tumor cell types.

Fig.1 Tumor Progression Models And Lineages.¹

Tumor Single Cell Sequencing Lineage Tracing Solutions

The technique of lineage tracing is a crucial analytical method for investigating the origin, development, and clonal evolution of tumor cells. With advancements in our single-cell sequencing technology and associated computational tools, these seq data have been effectively utilized for extracting cell lineage information. As a result, to get the most accurate information about your tumor cells, Creative Biolabs has established cost-effective and end-to-end tumor single-cell lineage tracing platforms. We can receive single metadata in many formats and have built a seamless single-cell assay pipeline. Our unique single-cell sequencing is operated by a team of PhD-level scientists. At single-cell lineage tracing analysis, we are committed to developing customized solutions for your unique projects, ensuring fast discovery times and unlocking valuable biological insights.

Fig.2 Combination of Single-Cell Genetic Lineage Tracing and Single-Cell Transcriptomics.²

Chief Services

• Single-cell Transcriptome Sequencing Lineage Tracing Service

Relying on a variety of commercial platforms, such as BD Rhapsody, 10x Genomics Chromium system, and the Illumina NGS platform, Creative Biolabs can achieve true tumor single-cell transcriptome sorting and labeling.

• Single-cell Assays For Transposase-accessible Chromatin (scATAC-seq) Lineage Tracing Service

Our scATAC-Seq services can simultaneously detect genome-wide chromatin accessibility across thousands of cells, revealing differences in chromatin accessibility within the nucleus at the single-cell level, and revealing epigenetic differences and dynamics in tumor cells to provide tumor lineage tracing data.

• Single-cell Tools-based Lineage Tracing Service

Single-cell technology has greatly improved the ability to trace the lineage of different tumor cells. From single-cell data describing these intermediate cell states, Creative Biolabs has developed several single-cell experimental tools and computational tools to reconstruct tumor cell state transition trajectories.

Advantages

• Professional sample handling ability
• Complete single-cell sequencing platform
• Stable and high-quality data quality
• Rich data analysis library.

Creative Biolabs is a leading CRO company that is dedicated to helping our worldwide customers shorten tumor single-cell lineage tracing analysis time. Additionally, experienced experts in our labs are also specialized in designing and performing high-quality custom single-cell sequencing assays. Please contact us with your project requirements, our experts are here to help.

References

1. Navin, N. E.; et al. Tracing the tumor lineage. Molecular oncology, 2010, 4(3): 267-283.
2. Kester, L.; et al. Single-cell transcriptomics meets lineage tracing. Cell stem cell, 2018, 23(2): 166-179.

For Research Use Only | Not For Clinical Use