T and B Cell-based ELISA Assay
A most elaborate study on epitope analysis can provide detailed information on the peptides in a protein antigen directly associated with T cell recognition and antibody binding. Using the versatile ELISA assay, Creative Biolabs offers cancer epitope analysis service by detecting the cytokines released by epitope-specific immune cells.
Enzyme-linked Immunosorbent Assay (ELISA) for Cytokines Test
Cytokines are soluble proteins of polypeptides or glycoproteins that are secreted by immune cells, including neutrophils, monocytes, macrophages, B cells, and T cells at a certain period, to modulate immune responses to stimulus. Enzyme-linked immunosorbent assay is the gold standard of immunoassays in the measurement of cytokines in plasma and cell cultures. The assay is based on antigen-antibody-specific binding and enzymatic reactions conjugated to the detection antibody.
ELISA-based Cancer Epitope Analysis Services at Creative Biolabs
The measurement of cytokines release is an effective approach to monitoring epitope-specific T-cell and B-cell responses. Now, we are proud to introduce the most popular ELISA-based method for cancer epitope analysis.
Workflow of the ELISA Assay
Work Steps of Sandwich ELISA
Fig.1 Protocol for sandwich ELISA.1
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Fixed capture antibody on a solid plate
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Add cell supernatant
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Add enzyme-labeled detection antibody
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Removing unbound enzyme conjugate and added substrate
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Detect signals by spectrophotometry
High-throughput ELISA Platform
✔ 96 well plates
✔ 384 well plates
✔ 1,536-well plates
Allowing multiple samples tested in one experiment.
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Cell in a single well can be scaled down to 104.
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A reduction in cell number by a factor of 4- to 50-fold over conventional assays.
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Screen a panel of peptides to select immunodominant epitopes.
The List of T and B Cell Cytokine Analytics
We have a wide range of ELISA kits to ensure that you can choose the proper candidates for your research.
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The List of T and B Cell Cytokine Analytics
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IFNg
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IL-5
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GM-CSF
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TNF
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IL-2
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IL-10
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IL-4
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TGFb
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IL-6
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IFNa
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IFNb
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IL-12
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IL-13
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IL-1b
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IL-8
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TNFa
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Granzyme B
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IL-9
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IL-21
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IL-22
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IL-23
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IL-17
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IL-17A
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Lymphotoxin A/TNFb
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CCL3/MIP-1a
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CXCL9/MIG
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CXCL10/IP-10
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CCL4/MIP-1b
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Lymphotactin
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Highlights
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High throughput
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Accurate and reliable data
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High-specific and sensitive results
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Optimized fast procedure and reduced assay time
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Standard Calibration to NIBSC
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Cost-effective
Related Cancer Epitope Analysis Services on Small Molecules
✔ T Cell-based Cytometric Bead Array Assay
✔ T and B Cell-based ELISPOT Assay
✔ T Cell-based High Throughput Multiplexed Assay
Creative Biolabs is skilled in employing various immunoassays for T-cell and B-cell on epitope screening. Please feel free to contact us for project quotations and get more detailed information.
References
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Lin, Hunter. Lateral Flow-Based Enzyme-Linked Immunosorbent Assay as a Point-of-Care Technique to Predict Type I Reversal Reactions in Lepromatous Patients. Biology in South India. 2016: 1-5.
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Howes, A. L.; et al. 3-Dimensional culture systems for anti-cancer compound profiling and high-throughput screening reveal increases in EGFR inhibitor-mediated cytotoxicity compared to monolayer culture systems. PloS one. 2014, 9(9): e108283.
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Henson, S.N.; et al. PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide libraries. Nat Protoc. 2023, 18: 396-423.
For Research Use Only | Not For Clinical Use
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