Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of the liver serum parameter alanine aminotransferase (ALT). As an end-to-end company offering high-quality analytical development and qualification services, we have the best advantages in assisting our worldwide customers.

Physiological Functions of ALT

ALT has diverse physiological functions including its obvious role in amino acid metabolism. ALT could catalyze the reversible transfer of L-alanine and α-ketoglutarate (a-KG) to pyruvate and glutamate in the citric acid cycle, which is the major energy-yielding metabolic pathway in cells with the liver being the principal site (Fig.1A). In another circulation between tissues, ALT located in hepatocytes converts alanine to pyruvate, a precursor of glucose. At the same time, ALT converts pyruvate to alanine in the muscle (Fig.1B). This glucose-alanine cycle, with the catalyzer ALT, existing between the liver and muscle is of great importance for glucose regulation and energy homeostasis.

Fig.1 Functions of ALT. (McGill, 2016)

Pathological Function of ALT

As a well-known hepatocellular leakage enzyme, ALT is produced mainly in the cells of the liver. The liver, as the largest solid organ in the body, is one most important tissue responsible for various metabolic processes, including the removal of toxins. If there is cell damage or cell inflammation, the ALT will leak out from the damaged cells into the bloodstream, leading to an abnormal concentration rise in the serum. Normal serum ALT levels are not reliable indicators of a healthy state, but elevated serum ALT levels are suggestive of hepatic inflammation. Therefore, serial measurement of serum ALT, as the most sensitive non-invasive marker, could be used to diagnose and monitor hepatocellular injury and disease activity.

ALT Production Measurement Services at Creative Biolabs

In the research of pharmaceutical and vaccine development, toxicity assessment by quantification of ALT, as a non-invasive biomarker, could help detect liver tissue damage. It could also help real-timely monitor the circulatory system in various pathological processes. At the same time, toxicity assessment by quantification of ALT may be used to screen and get the best effective candidates without severe side effects and avoid morbidity.

Considering its importance, ALT detection has been researched well by scientists of Creative Biolabs. With decades of in-depth study of this toxicity assessment area, we are the one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies.

Our techniques for the quantification of ALT in serum include spectrophotometric, chemiluminescence, fluorescence, and UV absorbance. Creative Biolabs offers advanced custom analytic services with the combination of our software and multiplex analyzers.

Features of Quantification of ALT Services

• Advanced technical platform with high sensitivity, accuracy, and repeatability
• Affordable price with the best quality
• Reliable lab report with timely update

Normally, ALT is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like Alkaline Phosphatase, Bilirubin, and Creatine Kinase. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your project. If you are interested in this, please feel free to contact us directly.

Reference

1. McGill, M.R. The past and present of serum aminotransferases and the future of liver injury biomarkers. EXCLI journal. 2016,15: 817.

For Research Use Only | Not For Clinical Use