Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of the serum parameter alkaline phosphatase (ALP). To meet the service you need, our highly skilled scientists are ready to get started on your projects.

Physiological Functions of ALP

The ubiquitous enzyme alkaline phosphatase (ALP) is widely distributed in the human liver, bones, intestines, kidneys, and placenta. As shown in Fig.1, ALP has been involved in the design of phosphorylate prodrugs, phosphate-functionalized drug delivery systems, and probes for diagnosis. It plays an integral role in the body's metabolism processes, especially within the tissue liver and the development processes in the skeleton. There are several isoenzymes of ALP with similar catalytic activities but different structural forms depending on the tissue producing it. Playing an important physiological role in dephosphorylating compounds, the ALP enzymes catalyze the hydrolysis of monoesters of phosphoric acid and the transphosphorylation reaction. As these reactions are involved in many biological functions like information storage and transfer, energy exchange, and membrane fluidity, the ALP enzymes hold key roles in many physiological processes.

Fig.1 Distribution of ALP as a target for drug delivery and diagnosis. (Le-Vinh, et al, 2022)

Pathological Function of ALP

ALPs are membrane-bound glycoproteins attached to the exterior of the plasma membrane and they are highly expressed in the extracellular space. In the serum, there are two types of ALP isozymes in the majority (>80%): skeletal and liver. Because of the widespread prevalence of ALP, serum ALP is regarded as a good biomarker in many diagnoses such as bone disorders, hepatitis, bone disorders, and cancers.

ALP Production Measurement Services at Creative Biolabs

In the research of pharmaceutical and diagnosis development, toxicity assessment by quantification of ALP, as a universal biomarker, could help detect liver diseases like hepatitis, cirrhosis, tumor, abscess, granulomas, or amyloidosis. ALP tests may also help real-timely monitor the circulatory system in various pathological processes. Considering its importance, ALP detection has been researched well by scientists of Creative Biolabs.

To qualify and differentiate the ALP isoenzymes, their properties like electrophoretic mobility, stability to denaturation by heat or chemicals, response to selected inhibitors, a high affinity for specific lectins, and immunochemical characteristics may be used in the immunoassay, isoelectric focusing, electrophoresis techniques, specific monoclonal antibodies, heating inactivation, and precipitation techniques. Creative Biolabs offers advanced custom analytic services with the combination of our software and multiplex analyzers. With decades of in-depth study of this toxicity assessment area, we are the one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies.

Features of Quantification of Alkaline Phosphatase Services

• Advanced technical platform with high sensitivity, accuracy, and repeatability
• Quantitative and qualitative project assessment
• Reliable lab report with timely update

Normally, ALP is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like ALT, Bilirubin, and Creatine Kinase. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your fitness project. If you are interested in this, please feel free to contact us directly.

Reference

1. Le-Vinh, B.; et al. Alkaline phosphatase: a reliable endogenous partner for drug delivery and diagnostics. Advanced Therapeutics. 2022, 5(2): 2100219.

For Research Use Only | Not For Clinical Use