Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of Total Protein. As an end-to-end company offering high-quality analytical development and qualification services, we have the best advantages in assisting our worldwide customers.

Introduction of Total Protein

Proteins as building blocks for organs, hormones, and enzymes, are essential for overall body health. Serum proteins include albumin, globulins, and many other proteins. After uptake and metabolism, the proteins left in the blood are filtered by the kidney, resulting in only a small amount of protein remaining in the urine. Total protein represents the total concentration of proteins presented in serum or urine body fluid. Abnormal levels of total protein could lead to unexpected weight loss, fatigue, edema, dehydration, bone marrow disorders, inflammatory disease, and some types of cancer. Because protein is related to several drug metabolism processes, serum or urinary total protein is one most sensitive and specific diagnostic biomarker for drug-induced hepatotoxicity, kidney toxicity along with other toxicity conditions.

Fig.1 The correlation of urinary total protein with glomerular injury. (Dieterle; et al., 2010)

Total Protein Services at Creative Biolabs

Measurement of the total protein concentration level in the initial stages or before treatment is a fundamental requirement to identify the potential risks of the progression to treatment-induced hepatotoxicity, the kidney toxicity. By comparing the concentration changes of total protein between the experimental group and the control group, we could timely monitor possible toxic effects caused by the metabolism of candidate drugs or vaccines. At the same time, total protein monitoring is necessary for dose optimization to manage protein level abnormalities and prevent the occurrence of toxicities.

Considering its importance in the evaluation of toxic effects, total protein measurement has been researched well by scientists of Creative Biolabs. The physiological fluid samples to determine total protein mainly include serum and urine, which could be collected by invasive or noninvasive methods. Measurement of total protein could be achieved by various methods, among which there are classical spectrophotometric methods, such as the Biuret method, Lowry method, Bradford method, and bicinchoninic acid (BCA) method. The protein standard is needed in these classical assays to avoid deviations caused by the variations in protein amino acid composition. In recent years, various modern detection biosensors, based on spectrophotometry, fluorometry, and chemiluminescence, have shown better performance with inherent specificity, simplicity, relatively low cost, rapid response, and proneness to miniaturization. Creative Biolabs provides total protein measurement suitable for our customers.

With decades of in-depth study of the toxicity assessment area, Creative Biolabs is one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies. With dedicated laboratory equipment and specially trained technicians, the detection procedures could be performed on fully automated analyzers, making it a routine service at Creative Biolabs.

Features of Quantification of Total Protein Services

• Advanced technical platform with high sensitivity, accuracy, and repeatability
• Quantitative and qualitative project assessment
• Affordable price with the best quality

Normally, total protein is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like bilirubin, alanine aminotransferases (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST). No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your project. If you are interested in this, please feel free to contact us directly.

References

1. Dieterle, F.; et al. Urinary clusterin, cystatin C, β2-microglobulin and total protein as markers to detect drug-induced kidney injury. Nature biotechnology, 2010, 28(5): 463-469.
2. Leca-Bouvier, B., & Blum, L. J. Biosensors for protein detection: a review. Analytical Letters. 2005, 38(10): 1491-1517.

For Research Use Only | Not For Clinical Use