CD3e Monoclonal Antibody (HJ21) (NHP-AB184)

• This product is an antibody that was generated by immunizing mouse against stimulated human leukocytes. It is used only in in vitro assays.

Detailed Product Description

• Introduction: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Conjugate: None
• Antibody Isotype: IgG2aκ
• Applications: IF

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Human; Monkey
• Immunogen: Stimulated human leukocytes

Product Property

• Purification: Protein G affinity chromatography
• Format: Liquid
• Concentration: 0.2 mg/mL
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Storage: Store at 4⁰C. Avoid freeze-thaw cycles.

Target Information

• Target: CD3e
• Clone: HJ21
• Clonality: Monoclonal
• Alternative Names: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
• Entrez Gene ID: 916