Cynomolgus Monkey IGFBP4 HEK293 Cell Overexpression Lysate (NHFF-0524-HX664)

• Creative Biolabs generates Cynomolgus monkey IGFBP4 overexpression cell lysate through engineered HEK293 cells. These over-expression lysates have been rigorously quality-controlled and are suitable for your Western blot experiments.

Detailed Product Information

• Source: From healthy Cynomolgus monkey
• Host: HEK293 cells

Technical Specifications

• Calculated MW: 27.4 kDa
• Apparent MW: 33 - 36 kDa
• Method of Preparation: This cell overexpression lysate was meticulously collected by a series of steps including the cell homogenization in cold optimized RIPA lysis reagent with multiple protease inhibitors, cell debris centrifugation, protein concentration determination by Bradford assay, lysate boiling in 1 x SDS loading buffer containing 5% b-mercaptoethanol, and lysate lyophilization.
• Lysis Reagent: An optimized RIPA lysis reagent is used in the preparation of this product. The specific ingredients are as follows: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, and 1mM PMSF.
• Attention before Use: Centrifuge briefly before use, then dissolve the pellet in 200μl pure water and boil for about 3 minutes.
• DNA Sequence: Met1-Glu258
• Tag: His
• Quality Control: The protein quality is verified by Coomassie blue staining after protein purification.

Storage and Shipping

• Storage: Usually stored at 4°C. Once re-dissolution, please aliquot this lysate into smaller sizes and transfer them into a -80°C refrigerator.
• Shipping: Ambient temperature or ice pack.