TLR4 Monoclonal Antibody (T3R12) (NHP-AB252)

• This product is an antibody that was generated by immunizing mouse against recombinant human protein. It is used only in in vitro assays.

Detailed Product Description

• Introduction: TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
• Conjugate: None
• Antibody Isotype: IgG2aκ
• Applications: Flow Cytometry; Func; IF; IHC-P

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Dog; Guinea Pig; Human; Monkey; Pig; Rat
• Immunogen: Recombinant human protein

Product Property

• Purification: Protein G affinity chromatography
• Format: Liquid
• Concentration: 0.2 mg/mL
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Storage: Store at 4⁰C. Avoid freez-thaw cycles.

Target Information

• Clone: T3R12
• Clonality: Monoclonal
• Alternative Names: CD284; homolog of Drosophila toll; hToll; lipopolysaccharide response; Toll-like receptor 4; Toll4
• Entrez Gene ID: 7099