AOC for DNA-PAINT Imaging

Antibody-oligonucleotide conjugate (AOC) is a new strategy and analysis tool in disease treatment and biological detection. The novel format is the combination of the specificity of antibodies and the programmability of oligonucleotide nanotechnology to provide a powerful ability in various applications including therapeutic, imaging, and detection.

Creative Biolabs has integrated multiple cutting-edge bioconjugation technologies and powerful antibody development platform. We provide a comprehensive portfolio of highly specific AOCs development services for customers worldwide. We have decades of experience in conjugate preparation and proprietary chemistry for all kinds of bioconjugation. Our state-of-the-art laboratories and scientists have delivered several antibodies, protein, and nucleic acid-based drug conjugates to customers. From novel linker design to conjugation of oligonucleotide, we strive to meet your needs and deliver the products on time with significant cost benefits.

Fig.1 Schematic representation of AOC use in (a) antibody arrays; (b) therapeutic; and (c) pretargeting applications. (Dovgan, 2019)

AOC Used for DNA-PAINT Imaging Application

DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) is a method for overcoming the conventional light microscopy (defined by the wavelength of the light source used), for super-resolution imaging, using a simple reagent-based approach. This method belongs to a family of super-resolution microscopy techniques. Molecules of interest in the cell are labeled with antibodies that are conjugated to single DNA strands. Complementary DNA strands are labeled with fluorophores and introduced in solution, they can transiently bind to their complementary strand on the antibody according to the binding kinetics. In this approach, the binding and unbinding of labeled DNA strands induce the same blinking effect that occurs stochastically from the dyes in traditional direct stochastic optical reconstruction microscopy (dSTORM).

AOCs used in DNA-PAINT are often based on the blinking of fluorophores (sequential activation), which permits them to be observed one at a time. This process of sparse activation is repeated until a sufficient number of position measurements are accumulated to allow the localization of the spatial coordinates of fluorophores with subdiffraction precision. DNA-PAINT achieves such “blinking” by the instantaneous binding of dye-labeled imager DNA strands to their target complementary docking DNA strands immobilized on the target of interest. In the process of immobilization, the melting temperature can be used to calculate accurate timing. When the fluorescence signals float freely in the solution, the fluorescence signals can be resolved spatially.

Fig.2 Mode of action of DNA-PAINT for the target imaging. (Dovgan, 2019)

Featured AOC Development Services at Creative Biolabs

Creative Biolabs has many years of experience in antibody development and antibody-related conjugation. We offer AOCs development service by implementing a state-of-the-art technology platform and expertise in providing high-quality conjugation strategies. Our AOCs conjugates are meticulously monitored and controlled according to stringent quality assurance and quality control standards.

Features of Our Services

• Full-spectrum of conjugation services
• Flexible technologies
• Optimized chemistries
• Guaranteed quality
• Cost and time savings

If you are interested in our AOCs development services, welcome to contact us for project quotations and more detailed information.

Reference

1. Dovgan, I.; et al. Antibody-oligonucleotide conjugates as therapeutic, imaging, and detection agents. Bioconjugate chemistry. 2019, 30(10): 2483-2501.