Viral Recombinant Protein Production Services
The demand for high-quality recombinant viral proteins in the field of virology research and diagnostics is continuously increasing. These proteins are key to understanding viral biology, pathogenesis, and host interactions, and are fundamental in developing vaccines, diagnostic tools, and therapeutic strategies. With advanced protein expression and purification techniques, Creative Biolabs is capable of efficiently producing a variety of recombinant viral proteins, covering multiple virus types, to meet the specific needs of different clients.
Fig.1 Design, purification, renaturation, and verification of the recombinant protein NMHC.1,2
What are Recombinant Viral Proteins?
Recombinant viral proteins are viral proteins manufactured in non-viral host cells through genetic engineering methods. These proteins are crucial components of viral particles, responsible for various biological functions such as virus attachment, entry into host cells, and replication. Recombinant proteins can be efficiently produced by inserting viral gene fragments into exogenous expression systems.
Recombinant Viral Protein Preparation Process at Creative Biolabs
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Step
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Item
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Specifics
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1
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Gene Cloning and Vector Construction
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Select the key protein gene of the target virus and clone it into an expression vector, such as a plasmid.
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2
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Host Cell Selection
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Choose the appropriate host cell based on the characteristics of the target protein.
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Mammalian cells (e.g., HEK293) are suitable for expressing surface proteins with complex structures and glycosylation modifications;
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Prokaryotic systems (e.g., E. coli) are more suited for expressing active viral enzymes;
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Insect cells can be used to produce proteins with simplified post-translational modifications.
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3
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Protein Expression
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Transfect the constructed expression vector into host cells to produce recombinant viral proteins.
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4
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Protein Purification and Analysis
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Extract and purify the target protein from host cells using various purification techniques. Perform biochemical and functional testing of the protein, such as checking protein structural integrity and activity.
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5
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Product Application and Development
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Use the obtained recombinant viral proteins for applications such as the development of diagnostic tools, virology research, or antibody development. For example, using SARS-CoV-2 RBD protein for serological testing, or developing antiviral drugs and vaccines.
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Advantages of Creative Biolabs Recombinant Viral Proteins
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Over a decade of vaccine development experience.
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A variety of ready-to-use recombinant viral proteins.
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Diverse recombinant viral protein purification techniques.
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Advanced equipment and technology to ensure high-purity products.
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Comprehensive support and customized solutions.
Value and Applications of Recombinant Viral Proteins
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Virology Research: Recombinant viral proteins are key tools in exploring how viruses invade cells and trigger disease processes. They provide a window into understanding the viral life cycle and pathogenic mechanisms.
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Drug Discovery Based on Viral Recombinant Protein: In the field of drug development, recombinant proteins are indispensable. They not only help scientists screen and optimize antiviral drugs but also play a crucial role in studying the mechanisms of virus-host cell interactions.
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Diagnostic Tool Development: The application of recombinant viral proteins is not limited to laboratory research. They are also widely used in developing diagnostic kits to detect specific viral infections, such as ELISA kits. These kits effectively aid in diagnosing viral infections by identifying specific viral antibodies or antigens.
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Vaccine Development: In modern vaccine R&D, recombinant viral proteins play a central role, especially in the development of subunit vaccines. These vaccines contain one or more viral proteins, rather than entire live virus particles, to provoke an immune response.
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Therapeutic Applications: In some cases, recombinant viral proteins and their derivatives themselves can serve as therapeutic agents, used to combat viral infections and treat other related diseases.
For years, Creative Biolabs has been committed to helping clients delve deeply into the field of infectious disease research. If you require specialized recombinant viral proteins, we invite you to contact us. Our team is dedicated to providing tailor-made solutions and expert support to meet your research needs in this critical area.
References:
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Li, Y.; et al. A recombinant protein containing influenza viral conserved epitopes and superantigen induces broad-spectrum protection. Elife. 2021,16;10.
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under Open Access license CC BY 4.0, without modification.
For Research Use Only. We do not provide direct services or products for patients.
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