Creative Biolabs is committed to offering top-notch viral vector design and synthesis services for a wide range of applications in virus infection research and diagnostics fields. As experts in the industry, we specialize in designing, constructing, and producing high-quality vectors, such as plasmids, lentiviruses, adenoviruses, and adeno-associated viruses (AAVs), tailored to meet all your experimental needs.
1. Vector design.
This involves designing the genetic material that the vector will carry. A vector is a type of virus that has been engineered for gene therapy by removing the pathogenic properties and using its ability to infect cells to deliver therapeutic genes. The design stage includes deciding on the types of genes to be delivered, the promoter sequences for the gene expression, and any regulatory sequences needed.
2. Plasmid construction and purification.
Plasmids are used to produce large amounts of the designed gene sequence. They also contain antibiotic resistance markers, which allow for easy selection of successfully transformed cells.
3. Transfection and virus production.
The purified plasmids are then introduced into a packaging cell line – cells that can produce the viral particles.
4. Purification and titration.
Viral particles are purified from the packaging cells involving cell lysis to release the viral particles, followed by a series of ultracentrifugation steps. The purified viral particles are then quantified by measuring the amount of viral DNA or RNA, or by measuring the infectious activity.
5. Quality control.
The final step involves verifying the purity, sterility, and absence of replication-competent particles of the completed batch of viral vectors.
Fig. 1 Viral vector expression systems.1
Our team of experienced geneticists and bioengineers utilize cutting-edge molecular cloning techniques to design and construct plasmid vectors with your gene of interest. We ensure high-quality, sequence-verified plasmids to meet your research needs.
Our customized lentiviral vector design and production services incorporate your desired gene into lentiviral vectors with high titers. Even for difficult-to-transfect genes, these lentiviral vectors are great instruments for steady gene delivery and expression.
Our team provides custom adenoviral vector design and production services for high-level transient gene expression. Adenoviruses are perfect tools for in vivo studies, allowing for efficient gene delivery to a wide variety of dividing and quiescent cell types.
We offer cutting-edge AAV vector design and production services. These vectors are optimal for stable, long-term gene expression in both dividing and non-dividing cells, making them a good alternative for in vivo investigations, particularly in neuroscience.
At Creative Biolabs, we are powered by technology, driven by knowledge, and guided by a commitment to help our clients succeed. Please contact us today for your viral vector design and synthetics service needs. Let's make your research a success!
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