Creative Biolabs, a world-leading service provider in the field of antibody discovery and generation, is proud to launch our high-quality in vitro diagnostic (IVD) antibody & immunoassay development services to global clients. Especially, we introduce Nestin as a potential marker of breast cancer.
Nestin (neuronal stem cell protein), encoded by the NES gene in humans, is a member of the class VI family of intermediate filament proteins. These intermediate filament proteins are expressed mostly in nerve cells where they are involved in the radial growth of the axon. Nestin is a major cytoskeletal protein expressed by neuronal precursors in the mammalian central nervous system (CNS). It has been shown to play a role in the regulation of the assembly and disassembly of intermediate filaments which is involved in remodeling of the cell together with other structural proteins. Furthermore, nestin expression has been widely used as a marker for multipotent neural stem cells (NSCs). Nestin knockout mice show embryonic lethality, with neuroepithelium of the developing neural tube displaying significantly fewer NSCs and much higher levels of apoptosis. These findings suggest that nestin is essential for the proper survival and self-renewal of NSCs.
Fig.1 Signaling pathways and cellular processes of nestin.1
Many studies have reported that nestin plays a role in the pathology of malignant diseases and is expressed in transformed cells of various human malignancies, suggesting a relationship between nestin expression and the clinical course of some diseases. Nestin is expressed in breast cancer stem cells and newly-formed tumor vessels. It is involved in tumor invasion and metastasis. In younger patients with breast cancer, nestin expression is significantly higher. In more aggressive breast cancer cell lines (MDA-MB-231 and BO2), the level of nestin protein is higher. Moreover, nestin immunohistochemical positivity is associated with poor clinical outcomes and decreased survival rates of breast cancer, suggesting nestin is a potential biomarker for breast cancer prognosis. Moreover, studies have reported the immunohistochemical detection of nestin expression may also be useful for the grading of astroglial tumors.
The improvement of disease detection methods can dramatically increase patient survival rates, which largely relies on the discovery of disease biomarkers. Nestin is one of the potential biomarkers for breast cancer diagnosis and prognosis. Creative Biolabs offers expertise in designing antibody-based kits and diagnostic assays to our global clients. With years of experience in antibody development, we can help with the development of antibodies with high affinity and specificity to generate a reliable immunodiagnostic kit. For more detailed information about what we offer for each stage of the development process, please click the links below:
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