Creative Biolabs offers exceptional dosing solutions analysis services for ADME. Our cutting-edge laboratories are furnished with the most advanced analytical technologies, guaranteeing accurate and dependable measurement of test compound concentrations. By employing advanced methodologies and rigorous testing protocols, Creative Biolabs guarantees accuracy and repeatability in every analysis we conduct.
High-Performance Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
Liquid chromatography (LC) is an analytical method that utilizes a liquid mobile phase to separate and analyze components within a mixture. In high-performance liquid chromatography (HPLC), the liquid solvent serving as the mobile phase is pressurized to flow swiftly through the column, facilitating swift and effective separation of the sample components. Mass spectrometer (MS) is an analytical tool used to detect and measure compounds in samples by determining their mass-to-charge ratio (m/z).
Fig.1 The data-dependent analysis of peptides in mixture using LC-MS/MS (part e).1
LC-MS/MS (liquid chromatography with tandem mass spectrometry) possesses powerful separation and selectivity capabilities. In LC-MS/MS, the sample is initially separated using liquid chromatography. Then, the eluted compounds enter the mass spectrometer, forming charged ions. The tandem mass spectrometer can be divided into MS1 and MS2. In the first mass spectrometer (MS1), ions are isolated and then directed into a collision cell where they undergo fragmentation. The fragmentation process results in the creation of product ions, which are subsequently separated and detected in the second mass spectrometer (MS2).
Through the integration of LC's high-resolution separation capabilities with tandem mass spectrometry's sensitive and selective detection, LC-MS/MS facilitates the precise identification and quantification of complex mixtures with exceptional sensitivity, accuracy, and specificity.
Dosing Solution Analysis by HPLC-MS/MS
Dosing solution analysis is conducted to confirm the concentration of the test compound in dosing solutions utilized in ADME-Tox or PK assays. HPLC-MS/MS is very useful in the quantitation study of compounds in dosing solutions. HPLC-MS/MS is suitable for various types of samples, and its particular advantage lies in handling complex samples or samples containing trace target compounds. The response is determined based on the peak area generated. Each concentration in the test is analyzed with three replicates during the assay.
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Through ongoing investments in research and development and the continual enhancement of our team's expertise, Creative Biolabs guarantees our position as a leader in ADME. Our steadfast commitment to quality, accuracy, and client contentment establishes us as a reliable partner for enterprises in search of dependable and exceptional ADME solutions.
You might be interested in these ADME-related services: Analysis of Drug Metabolic Stability, Kinetic Solubility Testing, Thermodynamic Solubility Testing. Additionally, don't hesitate to contact us at any time with any inquiries or requirements you might have.
Reference
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Duracova, M., et al. " Proteomic Methods of Detection and Quantification of Protein Toxins." Toxins 10.3 (2018): 99.
For Research Use Only | Not For Clinical Use
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