GM-CSF-modified whole tumor cells have been reported to induce strong and long-lasting systemic protection against the tumor challenge. With more than a decade of experience in the engineering of cell lines, Creative Biolabs offers innovative tools and services to engineer cancer cell lines with GM-CSF overexpression. Each project is customized by working directly with our team of highly trained scientists.

GM-CSF Functions in T cell Activation

Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first defined as a growth factor that induces the differentiation and proliferation of myeloid progenitors in the bone marrow. It is capable of affecting not only the phenotype of myeloid lineage cells but also T-cell activation through various myeloid intermediaries. Since lymphocytes generally do not express GM-CSF receptors, most of the demonstrated effects of GM-CSF on T cells are believed to be exerted indirectly through antigen-presenting cells (APCs).

• GM-CSF can drive the differentiation of bone marrow progenitor cells into dendritic cells and macrophages.
• GM-CSF stimulates monocytes/macrophages to secrete some pro-inflammatory cytokines (for example, IL-6, IL-23 and CCL17).
• GM-CSF can stimulate the proliferation and activation of monocytes/macrophages, DCs, T cells, neutrophils, and B cell.
• GM-CSF may directly influence B-cell phenotype and function including antibody production.

Fig.1 Immunobiologic effects of GM-CSF. (Kaufman, et al., 2014)

Studies Performed with GM-CSF Overexpressing Tumor Cells

Transfection of autologous or allogeneic tumor cells with the GM-CSF gene in preclinical models has demonstrated induction of cancer-specific antitumor immunity requiring both CD4+ and CD8+ cells mediated through dendritic cell stimulation. GM-CSF-modified cancer cells have been widely studied in various cancer treatments, including melanoma, prostate cancer, lung cancer, renal cell carcinoma, etc.

Engineering Cancer Cells with GM-CSF Overexpression Service

The modification of tumor cells used as tumor vaccines can improve the efficacy of anti-tumor therapy. As a globally leading supplier of bioreagents and CRO services for the biopharmaceutical field, Creative Biolabs provides comprehensive solutions for engineering cancer cell development. Our dedicated bioproduction cell line engineering team uses a state-of-the-art gene transfer platform to deliver your cancer cell engineering requirements with high precision and speed. Our experienced team will help determine the best-suited technology based on your project. Our capabilities also include functional studies involving bioassays, efficacy tests, toxicity assessment, further custom tune-up or validation, and more. Whether you work in drug discovery, or upstream, or downstream bioprocessing, we can help you to achieve considerable cost and time savings on the road to the clinic.

Our cell engineering services cover a range of different cancer cell lines, including:

Workflow

Creative Biolabs is now offering custom cancer cell engineering services addressing multiple components of the cell engineering workflow. If you have any questions, please don’t hesitate to contact us.

Reference

1. Kaufman, H.L.; et al. Current status of granulocyte–macrophage colony-stimulating factor in the immunotherapy of melanoma. Journal for immunotherapy of cancer. 2014, 2(1):1-3.

For Research Use Only | Not For Clinical Use