Genetically modified tumor cells represent one of the most effective cancer vaccine strategies. Cytokine gene transfer into weakly immunogenic tumor cells could enhance the generation of tumor-reactive T cells thus inducing antitumor efficacy. Creative Biolabs provides customized services for the development of IFN-γ gene-modified cancer cells. These gene-modified tumor cells can be applied in tumor immunotherapy.

IFN-γ Functions in T Cell Activation

IFN-γ is a pleiotropic cytokine and plays a critical role in orchestrating both innate and adaptive immune responses against viruses, bacteria, and tumors. It is produced primarily by activated T cells and NK cells. IFN-γ plays an important role in inducing and modulating an array of immune responses. It could regulate the relative abundances of different T cell populations either by actively stimulating some cell populations or by actively suppressing others.

• IFN-γ directly acts as a cytotoxic CD8 T cell differentiation signal, and it is essential for the induction of cytotoxic T cell precursor proliferation.
• IFNγ skews the helper T-cell response towards a Th1 profile, but inhibits regulatory T (Treg) cells, Th2 and Th17 differentiation.
• IFN-γ activates macrophages and makes them better able to mount an effective immune response.
• IFN-stimulates DCs, up-regulating pro-inflammatory factors.

Fig.1 IFN-γ interplay with immune cells. (Jorgovanovic, et al., 2020)

Studies Performed with IFN-γ Overexpressing Tumor Cells

• A study showed IFN-γ-modified nonimmunogenic tumor cells could elicit therapeutic T cells from draining lymph nodes capable of successfully treating established pulmonary metastases upon adoptive transfer.
• Several studies have demonstrated IFN-γ expressed on the transduced melanoma cells promoted antigen presentation and T-cell responses against the transduced tumor cells, leading to complete tumor regression in the tumor-bearing animals.
• Gene modification with IFN-γ gene can significantly enhance the expression of HLA class I molecules in hepatocellular carcinoma cell lines and may increase its immunogenicity.

Engineering Cancer Cells with IFN-γ Overexpression Service

IFN-γ gene therapy is considered a promising therapy for cancer treatment. IFN-γ-modified cancer cells have been used to induce the antitumor immune response. Creative Biolabs offers you state-of-the-art cancer cell line engineering and production services. With our custom service, you can get the optimal expression of IFN-γ gene on the host cell of your interest. Our team has accumulated a wealth of experience and utilizes our expertise to serve our customers with one-stop service, including gene synthesis, plasmid construction and preparation, packaging and transduction, and bioassays in one service package. We can help you advance your cell therapy product to clinical applications.

Our cancer cell engineering services cover a range of different cell lines. The following lists the available cancer cell types:

Partner with Creative Biolabs for tailored services and let us help you meet your unique research needs. If you have any questions, please don’t hesitate to contact us.

Reference

1. Jorgovanovic, D.; et al. Roles of IFN-γ in tumor progression and regression: a review. Biomarker research. 2020, 8: 1-6.

For Research Use Only | Not For Clinical Use