Supported by advanced technology and dedicated research teams, Creative Biolabs is dedicated to providing one-stop services for establishing cancer cell lines with customized genetic modifications at unbeatable prices with rapid turnaround. We have the ability to generate IL-12 gene-modified cancer cell lines for your basic research and clinical transformation. We believe you can achieve more on innovative research and get your next breakthrough cancer treatment with the help of Creative Biolabs.

IL-12 Functions in T Cell Activation

Interleukin-12 (IL-12) is a potent, pro-inflammatory type 1 cytokine and can potently activate T cells and NK cells. It plays a key role in inducing antitumor immune responses, including the induction of antitumor immune memory. IL-12 functions to activate and link the innate and acquired immune responses. IL-12 functions are listed below.

• IL-12 acts as a growth factor for activated NK and T cells, enhances the cytotoxic activity of NK cells, and favors cytotoxic T lymphocyte generation.
• IL-12 induces interferon-γ (IFN-γ) production by NK, T cells, B cells, dendritic cells (DC), and macrophages.
• IL-12 promotes the differentiation of naı̈ve CD4+ T cells into Th1 cells.
• IL-12 can up-regulate the production of IgG2a and IgG2b antibodies.

Fig.1 Schematic of the biological properties of IL-12. (Trinchieri, 2003)

Studies Performed with IL-12 Overexpressing Tumor Cells

• A study showed the IL-12-based tumor vaccine-induced expansion of tumor-specific interferon-gamma (IFN-gamma)-producing CD8(+) T cells in mouse disseminated A20 lymphoma model.
• A study indicated IL-12 gene-modified mammary sarcoma murine cell line could exert gene therapy in the tumor model by improving anti-tumor cellular immunity.
• A first clinical phase I study has demonstrated vaccination with IL-12 gene-modified autologous melanoma cells induced a heavy infiltration of CD4+ and CD8+ T lymphocytes and an increased antitumor immune response.

Engineering Cancer Cells with IL-12 Overexpression Service

Cytokine gene transfer into tumor cells has been shown to mediate tumor regression and antimetastatic effects in several animal models via immunomodulation. Focused on state-of-the-art cancer immunotherapy, Creative Biolabs has established an advanced platform to provide clients with IL-12 gene-modified cancer cell line services according to customized requirements. Our scientists are experts in various gene transfer techniques which allow us to achieve superior gene editing efficiency in a variety of cells, at a lower cost and in a shorter time.

We have the comprehensive capacity to provide customers with cell engineering services for various cancer cell lines. You can provide your cell lines or choose from our in-house collection. The following lists the available cancer cell types. If your desired cell is not found in our catalog, please consult with our scientists.

Scientists at Creative Biolabs love to contribute to your success with our expertise and full services. If you are interested in our services, please don’t hesitate to contact us.

Reference

1. Trinchieri, G. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nature Reviews Immunology. 2003, 3(2): 133-46.

For Research Use Only | Not For Clinical Use