The whole tumor cells can be genetically modified to express cytokines, chemokines, or costimulatory molecules, thus stimulating the immune response against cancer. Creative Biolabs provides tailored services for the construction of VEGF165 gene-modified cancer cells. Our services and expertise can help clients meet appropriate requirements to advance their cancer immunotherapy.

Functions of VEGF165

Vascular endothelial growth factor (VEGF) is a primary stimulus of angiogenesis in tumors and functions through binding VEGF receptor-2 (VEGFR2) and VEGFR1. It can increase vessel permeability and confer chemotaxis to monocytes. VEGF exists in multiple splicing isoforms including VEGF121, VEGF165, VEGF189 and VEGF206. Unlike human VEGF, mouse VEGF exists as three isoforms, VEGF120, VEGF164, and VEGF188. The VEGF165 isoform is a predominately expressed protein in the human body and is unique among the various VEGF isoforms in its ability to bind to the receptor neuropilin-1 (NRP-1), a coreceptor for VEGFR2.

Fig.1 Physiological pathways directed by the NRP/VEGF complex. (Talotta, 2022)

Engineering Cancer Cells with VEGF165 Overexpression Service

Studies show LL/2 lung carcinoma cell line engineering with VEGF164 overexpression induced the host’s protective immune response against parental tumor cells. VEGF164 overexpression probably attracted monocytes/macrophages into vaccinating inoculation sites, thus presenting antigens to Th-cells, enhancing CD8+ T cells’ recognition of tumor antigens, and inducing a host-specific immune response against tumor cells.

From this, we consider that lymphangiogenesis induction is a potential strategy as a novel immunotherapy. At Creative Biolabs, we develop genetically modified cancer cells with VEGFC overexpression to increase the killing of cancer cells by stimulating the immune system. Other than VEGFC overexpression, we also can engineer cancer cells with other cytokines and regulators overexpression. Our scientists have experience working with everything from easy-to-transfect cell lines to hard-to-transfect cell lines. We offer a variety of technology options and help choose the appropriate protocol depending on the cell line. Our services can offer researchers the advantages of comprehensive workflows that can begin from the desired gene sequence to the custom cell line, along with included verification procedures.

Creative Biolabs builds a team that provides comprehensive services to oncology professionals worldwide, covering every aspect of engineering cancer cells for cancer treatment. Our goal is to accelerate the development of cancer immunotherapies using technologies and expertise, and we have been researching over the years. We are proud to work alongside scientists worldwide to bring these tailor-made solutions to you.

Advantages

• One-stop solution that covers from design to verification
• Highly customizable solutions for engineering cancer cells
• Reliable services to make research easy

Creative Biolabs is committed to advancing next-generation cancer therapies covering various cancer types. Our flexible approach offers solutions to meet your project timeline and budget. If you are interested in your services, please feel free to contact us.

References

1. Kan, B.; et al. Irradiated VEGF164-modified tumor cell vaccine protected mice from the parental tumor challenge. Anti-Cancer Drugs. 2017, 28(2): 197-205.
2. Talotta, R. Impaired VEGF-A-mediated neurovascular crosstalk induced by SARS-CoV-2 spike protein: a potential hypothesis explaining long COVID-19 symptoms and COVID-19 vaccine side effects?. Microorganisms. 2022, 10(12): 2452.

For Research Use Only | Not For Clinical Use