Creative Biolabs offers comprehensive ADME measurement services designed to thoroughly assess the absorption, distribution, metabolism, and excretion of potential drugs in the context of cancer treatment. Our experienced team utilizes cutting-edge techniques to accurately evaluate drug metabolic stability, drug distribution, protein binding, drug-drug interaction, etc. Trust Creative Biolabs for unparalleled expertise in ADME studies tailored specifically for Cancer Immunotherapy.
CYP Induction Assays Using Hepatocytes
CYP induction assays are important in drug development and safety assessment. Creative Biolabs offers CYP induction assay service for evaluating the impact of test compounds on the activity of specific cytochrome P450 enzymes, such as CYP1A2, CYP2B6, and CYP2C9. This service is essential for understanding the potential drug-drug interactions and drug metabolism implications of new compounds.
Hepatocytes are known to contain high levels of CYP enzymes responsible for drug metabolism. At our lab, we offer a CYP induction measurement service using human hepatocytes. This service involves exposing the hepatocytes to various drugs or compounds and measuring the expression levels of CYP enzymes before and after induction.
Analysis Method
Our CYP induction assays are available in two methods.
In the context of CYP induction, qPCR can be used to measure the mRNA levels of specific CYP enzymes before and after treatment with a potential inducer. This allows researchers to determine if exposure to a test compound leads to an increase in the expression of CYP genes. Changes in mRNA expression levels can indicate transcriptional regulation of CYP genes.
In addition to mRNA analysis, enzyme activity assays can also be performed to directly measure the catalytic activity of CYP enzymes. Enzyme activity assays provide functional information on how these changes impact drug metabolism. These assays typically involve incubating hepatocytes with a CYP-specific substrate and measuring the production of metabolites using techniques such as high-performance liquid chromatography (HPLC) or mass spectrometry.
CYP Isozymes
There are multiple CYP isozymes, each with specific substrate preferences and functions. These isozymes are responsible for metabolizing a wide range of drugs and other compounds in the body. Our lab offers a CYP induction measurement service, which helps evaluate the potential of a compound to induce major CYP isozymes including:
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CYP1A2
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CYP2B6
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CYP3A
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CYP3A4
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CYP2C8
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CYP2C9
Q&A
1. Which isoforms are included in the CYP induction assay?
CYP1A2, CYP2B6, CYP3A, CYP3A4, CYP2C8, and CYP2C9 are all included in the CYP induction assay. For other isoforms, please contact us to inquire about availability.
2. Which detection method is better,qPCR or enzyme activity analysis?
Assays like qPCR can be used to measure the mRNA levels of specific CYP isozymes, providing information on their transcriptional regulation in response to various stimuli. Enzyme activity assays, on the other hand, can directly measure the functional activity of CYP isozymes in metabolizing specific substrates. However, a potential challenge of this method is the possible masking of a positive induction response in the presence of simultaneous inhibition. By combining mRNA analysis with enzyme activity assays, a more comprehensive understanding of CYP induction can be achieved.
If you are interested in assessing CYP induction in primary hepatocytes, please feel free to contact us for more information on our services and pricing.
For Research Use Only | Not For Clinical Use
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