Next-generation sequencing (NGS) has revolutionized cancer and clinical genomics with a cheap, powerful way to analyze the genome at breakneck speed. NGS also allow genome-wide studies in the form of mutation detection, tumor profiling, and disease surveillance with great efficiency. With such technology, clinicians and scientists can more easily determine the genetic causes of cancer and apply selective, individualized treatments.
Creative Biolabs, one of the world's leading oncology high-throughput NGS providers, is well-equipped with specialists in cancer genomics, bioinformatics, and molecular biology. Our complete genomics solutions are tuned to provide whole genome analysis for cancer research and treatment. We bring expertise and precision at all levels of the genomic pipeline, from sampling to reporting, to provide our clients with high-quality, proven insights for cancer studies and treatments.
What is NGS?
NGS revolutionizes genomics by enabling high-throughput, cost-effective sequencing of DNA and RNA, transforming research and clinical diagnostics. At Creative Biolabs, we utilize this powerful technology to support a wide range of applications, including cancer genomics, infectious disease studies, and biomarker discovery. We employ second-generation platforms such as Illumina for high-accuracy short-read sequencing and advanced third-generation technologies for long-read sequencing. Our services include sequencing-by-synthesis, single-molecule real-time sequencing, and nanopore-based approaches, ensuring customized solutions for complex research projects. With our comprehensive expertise, Creative Biolabs delivers accurate and actionable insights, driving innovation in genomics and beyond.
Core Steps in NGS
01 Sample Preparation
At Creative Biolabs, we start by extracting and fragmenting DNA or RNA into optimal sizes (200–400 bp). Our advanced techniques ensure high-quality nucleic acids, while platform-specific adapters enable efficient sequencing and sample multiplexing, critical for reliable results in genomics projects.
04 Library Construction
Using our state-of-the-art facilities, we construct robust DNA/RNA libraries through precise adapter ligation and PCR amplification. This ensures that each sample meets the required template quality and quantity, providing consistent data across high-throughput sequencing platforms.
02 Sequencing Reaction
Leveraging industry-leading platforms like Illumina, we perform sequencing-by-synthesis to capture high-throughput, base-by-base data with unparalleled accuracy. Our meticulous processes ensure that every read contributes to meaningful genomic insights, tailored to our clients' needs.
03 Data Analysis
Our bioinformatics experts process raw sequencing data through rigorous quality control, reference genome alignment, and advanced variant annotation. At Creative Biolabs, we transform raw data into actionable insights, empowering researchers to advance cancer genomics and personalized medicine.
Sequencing Platforms
Creative Biolabs offers multiple leading sequencing platforms to serve the varied demands of cancer genomics. Each platform has different strengths in terms of throughput, precision and pricing, enabling individual solutions for patient genome sequencing. Below is a comparative table of the sequencing platforms used by Creative Biolabs, illustrating their specifications and clinical relevance in oncology applications:
Sequencing Platform
Technology
Read Length
Throughput
Key Applications
Illumina HiSeq X
Paired-End Sequencing
151bp
High
Large-scale genome sequencing, mutation profiling
HiSeq2000/MiSeq
Paired-End, Mate Pair
75-150bp
Medium
Targeted cancer panels, RNA-Seq
GS-FLX Titanium/Plus
Shotgun, Mate Pair
400-800bp
Lower
Specialized applications requiring long reads
Relevant Genome Analysis Services
Genotyping Service
Creative Biolabs (CBL) offers a cutting-edge genotyping platform to support genomic analysis for cancer patients. This service enables health professionals to examine multiple SNPs across thousands of samples simultaneously, identifying genetic variants potentially linked to cancerous mutations or genetic traits.
Bioinformatics Analysis
CBL provides in-depth bioinformatics, including gene expression profiling from microarrays to qPCR and complex analysis of next-generation sequencing (NGS) data. Even for bulk regions with repeated reads, we can still provide the most complete and accurate genomic sequence using our state-of-the-art technologies. This means the service is equipped to handle repetitive regions, ensuring comprehensive genomic sequencing. Additional bioinformatics offerings include:
Gene Annotation: Genetic testing and functional assignment for detailed phenotype insights.
Modification Analysis: Detection of modified bases via polymerase kinetics.
Creative Biolabs offers targeted genome editing services for immuno-oncology research, utilizing advanced multiple genome-editing technologies to enhance cancer immunotherapies. These services focus on precise modifications to immune cells and tumor targets for improved therapeutic outcomes.
With over a decade of experience, CBL has cultivated global relationships, advanced infrastructure, and interdisciplinary collaborations. This foundation allows CBL to deliver integrated services from initial sequencing to advanced bioinformatics, supporting tailored solutions for clients worldwide. For more details, please feel free to contact us or send us a query directly.
Gene Expression Profiling
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