Shotgun Metagenomic Sequencing (MGS) for GutMicrobiota Genomics Analysis

Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype. Shotgun metagenomic sequencing (MGS) is a relatively new and powerful environmental sequencing approach that provides insight into community biodiversity and function. Creative Biolabs offers shotgun MGS service to characterize gut microbiota communities, enabling precise sub-species/strain-level and functional annotation of the microbiome.

Shotgun MGS

Shotgun MGS is suited for comprehensively sampling all genes in all organisms present in a given complex sample, which enables microbiologists to evaluate bacterial diversity and detect the abundance of microbes in various environments. Moreover, shotgun MGS also provides a means to study unculturable microorganisms that are otherwise difficult or impossible to analyze.

Compared to capillary sequencing or PCR-based approaches, next-generation sequencing (NGS) allows researchers to sequence thousands of organisms in parallel. With the ability to combine many samples in a single sequencing run and obtain high sequence coverage per sample, NGS-based metagenomic sequencing can detect very low abundance members of the microbial community that may be missed or too expensive to identify using other methods.

Fig.1 Shotgun metagenome sequencing analysis of four mangrove root-compartments microbiota. (Zhuang, 2020)

Applications of Shotgun MGS

• Microbiome biomarker discovery
• Research and discovery across a wide variety of human health areas such as gut, oral and skin health
• Clinical study analysis of antibiotic resistance genes
• Clinical study analysis for linking the microbiome to clinical outcomes in drug, probiotic and nutritional intervention studies
• Clinical study analysis of potential microbiome factors determining responders and non-responders
• Microbiome data mining for identifying disease-associated strains
• Engraftment and colonization analysis for fecal microbial transplant (FMT) studies and probiotic studies

Our Service

• Sample Preparation
  Creative Biolabs offers DNA extraction for metagenomics projects, performed under highly controlled and traceable conditions in our advanced and certified laboratory.
• Sequencing
  DNA sequencing at Creative Biolabs is performed using Illumina-based shotgun metagenomics sequencing at highly competitive prices.
• Data Analysis
  Creative Biolabs has rich metagenomics data analysis experience with advanced bioinformatic approaches:

• Custom Solutions
  Creative Biolabs offers de novo discovery of microbes based on the gut samples from your study. Besides, we also identify new cohort-specific species for describing the full microbial diversity of your samples.

If you are interested in our shotgun metagenomic sequencing service, please do not hesitate to contact us for any discussion.

Reference

1. Zhuang, W.; et al. Diversity, function and assembly of mangrove root-associated microbial communities at a continuous fine-scale. NPJ Biofilms Microbiomes. 2020, 6(1): 52.

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