Shotgun Metagenomic Sequencing (MGS) for GutMicrobiota Genomics Analysis

Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype. Shotgun metagenomic sequencing (MGS) is a relatively new and powerful environmental sequencing approach that provides insight into community biodiversity and function. Creative Biolabs offers shotgun MGS service to characterize gut microbiota communities, enabling precise sub-species/strain-level and functional annotation of the microbiome.

Shotgun MGS

Shotgun MGS is suited for comprehensively sampling all genes in all organisms present in a given complex sample, which enables microbiologists to evaluate bacterial diversity and detect the abundance of microbes in various environments. Moreover, shotgun MGS also provides a means to study unculturable microorganisms that are otherwise difficult or impossible to analyze.

Compared to capillary sequencing or PCR-based approaches, next-generation sequencing (NGS) allows researchers to sequence thousands of organisms in parallel. With the ability to combine many samples in a single sequencing run and obtain high sequence coverage per sample, NGS-based metagenomic sequencing can detect very low abundance members of the microbial community that may be missed or too expensive to identify using other methods.

Shotgun metagenome sequencing analysis of four mangrove root-compartments microbiota. Fig.1 Shotgun metagenome sequencing analysis of four mangrove root-compartments microbiota. (Zhuang, 2020)

Applications of Shotgun MGS

Our Service

  • Multi-omics integration
  • Advanced statistical modelling
  • Machine learning
  • Antibiotic resistance genes
  • SNV-based strain tracking
  • Responder/non-responder models
  • FMT/probiotic persistence probability
  • Phage discovery
    and more...

If you are interested in our shotgun metagenomic sequencing service, please do not hesitate to contact us for any discussion.

Reference

  1. Zhuang, W.; et al. Diversity, function and assembly of mangrove root-associated microbial communities at a continuous fine-scale. NPJ Biofilms Microbiomes. 2020, 6(1): 52.

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