T Cell-based Bioassay
Creative Biolabs provides a wide range of cancer epitope analysis assays to characterize tumor antigens. We now offer a T cell-based bioassay service to identify potential T cell epitopes by assessing the cytokines release category and levels elicited by epitope stimulation.
The Function of Cytokine Release Assay
Cytokines are the small molecules produced by immune cells engaged in transporting information between cells. Nature T cells are activated by the interaction between the T cell receptor and peptide-MHC complex, initiating cytokine secretion and specific cytotoxicity. Cytokine release assay has become a typical approach for evaluating risks and hazards of immunomodulatory cells and antibodies-induced cytokine release syndrome and predicting immunotoxicity. While cytokine release assay was originally used to evaluate drugs with known immunomodulatory function, the cytokine release bioassay has also been adopted for characterizing cell responses associated with other components of a molecule.
Fig.1 T cell-secreted cytokines.1
How Do We Use T Cell Bioassay for Epitope Screening?
By detecting cytokines release from human T cells cultured in vitro with different cancer epitopes, Creative Biolabs apply the assay to screen epitopes with anticipated agonist potential for inducing significant T cell immune response in vivo.
Workflow
Service Specifications of Our Bioassay
Various types of cytokines are produced from T cells after cancer antigen stimulation. The following kinds of cytokines are often used to monitor specific T cell responses.
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GM-CSF
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IFN-γ
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IL-10
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IL-2
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IL-3
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IL-4
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IL-5
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TNF
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IL-6
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IL-8
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IL-1β
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IL-12
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IL-17A
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MIP-1α
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TNF-α
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Available Technologies
We provide our clients with various methods for measurement, including but not limited to below. Creative Biolabs promises to provide a sensitive and reliable measurement of these cytokines. We are also pleased to develop tailored methods for your samples and objectives.
Highlights
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Advanced technologies and equipment
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Multiple validated reagents and assay kits
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Sensitive, accurate, precise cytokine release information
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Integrated data and scientific analysis report
Online Consultation
Creative Biolabs is devoted to accelerating your research with various versatile approaches. Our in-house experts on T-cell assay will partner with you throughout the whole process. If you are interested in any T-cell assay, please feel free to contact us to learn how we can be involved in your projects.
Related Services
T Cell-based Intracellular Staining Assay
At Creative Biolabs, intracellular protein markers, such as, perforin, granzyme B, and granzyme A, release from epitope-specific T cells can be monitored by intracellular staining assay.
References
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Yu, S. L.; et al. Immunopathological roles of cytokines, chemokines, signaling molecules, and pattern-recognition receptors in systemic lupus erythematosus. Clinical & developmental immunology. 2012: 715190.
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van Daalen, K. R.; et al. Modulation of Inflammation by Extracellular Granzyme A. Frontiers in Immunology. 2020, 11: 931.
For Research Use Only | Not For Clinical Use
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