T Cell-based In Vitro Assay
Creative Biolabs has developed a variety of T and B cell-based assays to analyze cancer antigens and identify immunodominant epitopes based on extensive, versatile techniques. The in vitro assay is a well-established and reproducible method for characterizing epitope-induced T cell responses, such as cytotoxicity, cell proliferation, and suppression function. Based on your sample, our experienced scientists can provide proper assays to accelerate your target-mediated immunotherapy development.
Cytokine release is a crucial signature of activated T cells and can be used to screen the cancer epitope that can induce immune responses. ELISA assay is typically applied to measure cytokine production quantitatively. With access to various commercial ELISA kits and high-quality antibodies, we provide a comprehensive profile of cytokine secretion activities.
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Techniques
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Functions
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Advantages
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Limitations
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ELISA
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Detect Cytokine Secretion
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Sensitive
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Accurate
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Widely Used
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Laborious
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Limited Detection Range
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Advanced ELISA
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Detect Cytokine Secretion
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High Throughput
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Greater Sensitivity
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Wide Range of Detection Limits
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Free of Washing Steps
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None
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The ELISA-related techniques include but are not limited to the following.
Fig.1 CD4+ T cells involved in antitumor immunity.1
Cell proliferation is the phenomenon of the clonal growth of T and B cells resulting from antigen stimulation and is one of the measurement markers of successful cell activation.
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Technology
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Principle
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Correlate Techniques
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Advantages
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Limitations
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CFSE Assay
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Reduced CFSE Signal as Cell Division
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Flow Cytometry
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Simple to Perform
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Relatively High Throughput
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Non-radioactivity
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May Influence Cell Viability
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3H-thymidine Assay
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Incorporate in Dividing Cells
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Liquid Scintillation Counter
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High Throughput
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Sensitive
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Radioactive
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BrdU Assay
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Incorporate in Dividing Cells
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Flow Cytometry
Colorimetric Method
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Specific and Direct
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High Specificity
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Radioactive
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51Cr Assay
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Chromium-51 Release
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Gamma Counter
Liquid Scintillation Counter
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Sensitive
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Reflect Cytotoxicity
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Radioactivity
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Regulatory T cells (Treg) are important immune cells that can control immune activation, maintain the balance of the host immune system, and prevent self-immune responses. It has been demonstrated that antigen-induced Treg cells with higher CD25 expression are derived from the same clone with corresponding CD25low effector T cells (Teff) and possess the same epitope-specific TCR sequences.
At Creative Biolabs, the epitope-specific Treg and Teff cells can be analyzed and sorted from epitope-peptide stimulated T cells using the flow cytometric technique. For the assessment of T cell suppression, cytokine release assay (IL-2, IL-10, and IFNg) and 3H-thymidine- and CSFE-based cell proliferation assays are available.
Contact Us
Our experts are pleased to apply our professional knowledge to your program and meet your special requirements. Want to explore cancer epitopes using these versatile in vitro assays? Please contact us by email.
Reference
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Kim, H. J.; Cantor, H. CD4 T-cell subsets and tumor immunity: the helpful and the not-so-helpful. Cancer immunology research. 2014, 2(2): 91-98.
For Research Use Only | Not For Clinical Use
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