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- This product is an antibody that was generated by immunizing rabbit against amino acids 73 - 87 of GAPDH (Human). It is used only in in vitro assays.
Detailed Product Description
- Introduction: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling.
- Conjugate: None
- Applications: IHC; WB
Technical Specifications
- Host Species: Rabbit
- Species Reactivity: Gibbon; Gorilla; Human; Monkey; Orangutan
- Immunogen: Amino acids 73 - 87 of GAPDH (Human)
Product Property
- Purification: Protein G Column
- Format: Liquid
- Concentration: 1 mg/mL
- Buffer: PBS, 0.05% BSA, 0.05% sodium azide
- Storage: Short term: 4˚C. Long term: Store at -20˚C.
Target Information
- Clonality: Polyclonal
- Alternative Names: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPD; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
- Entrez Gene ID: 2597
Downloadable Resources
FAQs
Customer Reviews
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Q 1: How specific is the GAPDH polyclonal antibody to GAPDH without cross-reacting with other proteins?
A: Rabbit GAPDH Polyclonal Antibodies are designed with high specificity to target the protein of GAPDH and have minimal cross-reactivity with any other substances. In addition, this product is tested for specificity by carefully selecting and screening during production. Avoid repeated freeze/thaw cycles. Minimize cross-reactivity with other proteins by the rigorous test using various species and tissues.
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Q 2: For which applications (e.g., Western blot, immunofluorescence) has the antibody been validated, and what are the recommended conditions?
A: This antibody is its application validated. For example, in the use of Western blot and immunofluorescence tests, it was These antibodies are validated against a wide range of applications: Western blot, IHC, among many others. Their usage, in terms of dilution ratios and incubation times that are recommended, is given huge consideration, many having gone through big optimization studies, in order to have the best performance by these antibodies in these assays.
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High Specificity
I recently used the GAPDH polyclonal antibody from Creative Biolabs to carry out Western blot analysis in my research of cell biology. It worked with high specificity, showing clear bands with almost no background. The technical support was also very good, offering optimized protocol tips. -
Excellent Performance
GAPDH from Creative Biolabs has proven to be a valuable reagent in our immunofluorescence studies. It produced both consistent and excellent performance over a variety of experiments, providing great assistance to our research findings in cellular metabolism.
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