DNA Library Prep - Creative Biolabs

DNA Library Prep

Overview Workflow Features Applications Products Customer Rreviews Resource

Overview

Creative Biolabs offers an advanced DNA Library Preparation service designed to convert genomic DNA into a sequencing-ready format, ensuring high-quality data output for next-generation sequencing (NGS) applications. This service is crucial for achieving reliable and comprehensive genomic analyses, directly impacting the depth and accuracy of sequencing results.

Workflow

The DNA Library Preparation workflow at Creative Biolabs includes the following key steps:

Fragmentation: Genomic DNA is fragmented into smaller, uniform pieces using physical or enzymatic methods, essential for generating high-quality libraries.
End Repair and A-Tailing: DNA fragments undergo end repair to create blunt ends, followed by the addition of an adenine (A) nucleotide to the 3' ends, preparing them for adapter ligation.
Adapter Ligation: Sequencing adapters, which contain platform-specific sequences and unique barcodes for sample identification, are ligated to the DNA fragments.
Amplification: Adapter-ligated fragments are enriched through PCR, increasing the quantity of DNA and ensuring the library is suitable for sequencing.
Quality Control: Rigorous quality control steps, including fragment size assessment and DNA quantification, ensure the library's integrity and readiness for sequencing.

Key Features and Benefits

Precision and Accuracy: Ensures consistent fragment sizes and accurate adapter ligation, resulting in high-quality sequencing data.
Platform Compatibility: Adapters are compatible with various NGS platforms, enhancing versatility.
Efficiency: A streamlined workflow minimizes preparation time and reduces the risk of sample loss.
Scalability: Suitable for a wide range of sample types and quantities, from low-input to high-throughput projects.
Flexibility: Sample prep kits are available for a broad range of input amounts, from pg to μg of DNA.

Applications

Genomics: Ideal for whole-genome sequencing (WGS) and targeted sequencing, helping identify genetic variations, mutations, and structural changes.
Transcriptomics: Supports RNA sequencing (RNA-Seq) to analyze gene expression profiles and discover novel transcripts.
Epigenomics: Facilitates bisulfite sequencing for studying DNA methylation patterns and understanding epigenetic regulation.
Metagenomics: Enables analysis of microbial communities in various environments, including human microbiomes and environmental samples.

At Creative Biolabs, DNA Library Preparation is a cornerstone of successful NGS projects, providing researchers with reliable and reproducible sequencing results. Our services support groundbreaking discoveries and innovations in cancer. Trust Creative Biolabs to meet your DNA library preparation needs, contact us for more information and a detailed quote.

Products

Cat	Product Name	Size	Application
	SuD DNA Adapters set1/2 for Illumina®		DNA sequencing
	SuD DNA Adapters set3-6 for Illumina®	20 μL each	DNA sequencing
SD301	SuD AmpSeq Library Prep Kit	24 rxn	DNA sequencing
SD401	SuD AmpSeq Cancer HotSpot Panel	24 rxn	DNA sequencing
SD501	SuD AmpSeq Adapters 1-12 for Illumina®	24 rxn	DNA sequencing
SD601	SuD AmpSeq Adapters 1-12 for Ion Torrent	24 rxn	DNA sequencing
SD701	SuD Mate Pair Library Prep Kit for Illumina®	48 rxn	DNA sequencing
SD801	SuD Nano DNA Library Prep Kit for Illumina®		DNA sequencing
	SuD Universal DNA Library Prep Kit for Illumina®		DNA sequencing
SD1001	SuDPrep Index Kit V2 for Illumina®	96 rxn	DNA sequencing
SD1101	SuDPrep Index Kit V3 for Illumina®	384 rxn	DNA sequencing
	SuDPrep Index Kit V4 for Illumina®	384 rxn	DNA sequencing
	SuDPrep Dual Index Sequencing Primer Box for Illumina®	1 rxn	DNA sequencing
	SuDPrep DNA Library Prep Kit V2 for Illumina®		DNA sequencing
SD1500	SuDPrep Fast DNA Library Prep Set for Illumina®		DNA sequencing
SD1600	SuDPrep Fast DNA Library Prep Set for Ion Torrent		DNA sequencing

Customer Reviews

Specificity:
"The bead-based technology in the DNA Prep kit ensured high specificity, minimizing off-target effects and providing clean, reliable sequencing results."
Sensitivity:
"The DNA Library Prep Kit showed remarkable sensitivity. Even with minimal input DNA, it produced high-quality libraries, ensuring all significant variants were detected."
Accuracy:
"Using the DNA Library Prep Kit, we achieved highly accurate sequencing data. The results matched our expectations, confirming the reliability of the kit in various applications."
Stability:
"The DNA Library Prep Kit demonstrated exceptional stability, maintaining consistent performance across multiple runs and different sample types, including FFPE samples."
Customer Support:
"The customer support for the Seq Kit was outstanding. They provided prompt and comprehensive assistance, ensuring we could troubleshoot and optimize our experiments efficiently."