Whole Transcriptome Sequencing (WTS) Service for Cancer

Transcriptome Background Services Workflow Related Services Features Q&As Resource

Whole transcriptome analysis has been an important tool in solving biological issues and understanding the molecular mechanisms of many diseases including human cancers. Whole transcriptome sequencing (WTS) is used to reveal the presence and quantity of RNA, in a biological sample under specific conditions. Cancer WTS service in Creative Biolabs enable discovery and profiling of RNAs without prior genome annotation. Our seasoned scientists provide the most accurate detection and quantification of rare RNA sequences and variants.

What is a Transcriptome?

Whole Transcriptome Sequencing (WTS) Service (Creative Biolabs Authorized) Fig 1. Scatterplot of expression of transcripts identified by whole-transcriptome sequencing (Chow et al. 2012).

The transcriptome is the entire set of RNA transcripts in a given cell for a specific developmental stage or physiological condition. RNA is a polymeric molecule implicated in various biological processes, such as the coding, decoding, regulation, and expression of genes. RNA molecules include mRNAs, long non-coding RNA (lncRNAs), circular RNA (circRNAs), microRNA (miRNA), message RNA (mRNA), transfer (tRNA) and ribosomal ribonucleic acid (rRNA).

The Background of Transcriptome Sequencing (RNA-seq)

RNA-seq is an important tool for interpreting the functional elements of the genome as well as for understanding the underlying mechanisms of diverse diseases, especially cancers. Microarray-based gene expression approaches have been used for high-throughput large-scale RNA-level studies, such as to identify differentially expressed genes. However, the application of microarray technologies has been limited by their hybridization-based nature. Advances in massive parallel DNA sequencing technologies have enabled RNA-seq by sequencing of cDNA. RNA-seq is a revolutionary tool with better resolution and higher reproducibility that offers many advantages over the traditional microarray technologies. RNA-seq can be used to extend our knowledge of alternative splicing events, novel genes and transcripts, and fusion transcripts. RNA-seq can also help to understand molecular mechanisms and then it tells about signaling pathways which controls embryonic development. The transcriptome data give researchers a good place to start searching for a newly found gene's function.

Cancer WTS Service at Creative Biolabs

Creative Biolabs offers a suite of services for cancer research, including library preparation, high-throughput sequencing and bioinformatics analysis. The tumor and tumor microenvironment are closely related and interact constantly in patients with cancer. To comprehensively understand the tumor microenvironment, our scientists can provide high-quality tumor microenvironment analysis service by RNA-seq.

Workflow of Cancer WTS Service at Creative Biolabs

  • Cancer WTS workflow. (Creative Biolabs Original)
    Total
    RNA
  • Cancer WTS workflow. (Creative Biolabs Original)
    Removal of rRNA
  • Cancer WTS workflow. (Creative Biolabs Original)
    cDNA synthesis
  • Cancer WTS workflow. (Creative Biolabs Original)
    Library preparation
  • Cancer WTS workflow. (Creative Biolabs Original)
    Sequencing
  • Cancer WTS workflow. (Creative Biolabs Original)
    Data
    analysis

Cancer WTS Service in Creative Biolabs Include but Are Not Limited to:

Key Features and Advantages of Cancer WTS Service Including but Not Limited to:

  • Investigate all RNAs, known and unknown
  • Detect novel and rare transcripts and transcript variants
  • Long read lengths for full-length transcripts, including low expressed transcripts.
  • Allowing the study of isoforms, splice variants and fusion transcripts

Whole transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to cancers. Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is dedicated to providing the best cancer WTS service and WTS bioinformatics analysis with our latest technologies.

Please contact us for more information and a detailed quote.

Q&As

Q: What types of cancer samples can be analyzed using WTS?
A: WTS can be performed on various sample types, including fresh frozen tissues, formalin-fixed paraffin-embedded (FFPE) tissues, and liquid biopsies. This flexibility makes it suitable for a wide range of cancer research and clinical applications.
Q: What are the data analysis steps involved in WTS?
A: Data analysis involves quality control, alignment to reference genomes, transcript quantification, differential expression analysis, and identification of novel transcripts and gene fusions. Advanced bioinformatics tools are used to ensure accurate and comprehensive results.
Q: How does WTS complement other genomic analyses like WGS?
A: WTS complements Whole Genome Sequencing (WGS) by providing insights into gene expression and transcriptomic alterations that are not captured by DNA sequencing alone. Together, they offer a complete view of the genomic and transcriptomic landscape of cancer.
Q: What is the importance of quality control in WTS?
A: Quality control is critical in WTS to ensure the integrity and purity of RNA samples, which affects the accuracy and reliability of sequencing data. This includes assessing RNA quality before library preparation and conducting quality checks throughout the sequencing process.
Q: Can WTS be used for studying tumor microenvironment interactions?
A: Yes, WTS can analyze the gene expression profiles of both cancer cells and surrounding stromal and immune cells. This helps researchers understand the interactions within the tumor microenvironment and identify potential therapeutic targets involved in these complex interactions.

Resources

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Note: Our sequencing services are for Research Use Only. Not For Clinical Diagnosis.
Related Services:
  1. Whole Genome Sequencing (WGS) Service for Cancer
  2. Whole Exome Sequencing (WES) Service for Cancer
  3. Targeted Sequencing Service for Cancer
  4. Immune Repertoire Sequencing (Rep-Seq) Service for Cancer
  5. Epigenomics Service
  6. Three-dimensional (3D) Genomics Service
  7. Metagenomics Sequencing Service for Cancer
  8. ctDNA Sequencing
  9. Single Cell Squencing for Cancer
  10. Spatial Transcriptome Sequencing for Cancer
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