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Phage Display for Protein Engineering

Creative Biolabs provides customers different phage display systems for protein engineering, suitable for screening and selection of high affinity target-binders. The phage display systems can be used in studies including Evolution of Binding Agents, Improvement of Protein Stability and Folding, Identification of Natural Protein–Protein Interactions, Specificity Profiling of Peptide-Binding Modules and Mapping of Binding Energetics.

Phage display has been used as an efficient method for engineering synthetic binding proteins and their diverse applications. Phage display, as the long-lasting molecular display technique during the last two decades, is based on the expression of the recombinant peptide or protein fused with a coated protein of bacteriophage particles with the encoding DNA. In phage display, a phenotype-genotype linkage has been established between each displayed molecule and the encoding DNA, which allows rapid identification of target-binding sequences by an in vitro selection process. As proteins and peptides are displayed on the surface coat proteins, they can be selected for binding and enriched by several rounds of selection. This technique also permits selection for binding to nonnatural compounds. Thus, phage display represents a dominant method for the engineering of binding proteins with novel functions. Besides that, it can be also used in the rapid and quantitative analysis of binding energetics across protein interfaces.

High-affinity binding proteins can be selected through in vitro binding to an immobilized target molecule from a library of displayed proteins, whose sequences can be rapidly deduced by DNA sequencing. Repertoire selection has been successfully achieved by phage display technology, especially for the directed evolution of molecular interactions, and the isolation of peptide hormone mimics or specific antibodies directly from repertoires of human V-genes. Recently, the proximal display of both substrate and enzyme on the phage particle using phage display has been adapted for the selection of catalytic activity of proteins as well. We can phage display your proteins, and subsequently study their functions with carefully designed experimental procedures.

Our phage display systems include but are not limited to:

  1. New phage display systems with alternative secretion pathways
  2. Synthetic antibodies
  3. Engineering binding proteins of scaffolds
  4. Combinatorial analysis of energetics across the binding interfaces


Figure 1. Schematic view of a filamentous phage particle. (Matthias Paschke, 2006)


Figure 2. Binding protein selection from phage display libraries. (Sachdev S Sidhu et al., 2007)

References

  1. Matthias Paschke. Phage display systems and their applications. Appl Microbiol Biotechnol 2006, 70: 2–11.
  2. Sachdev S Sidhu, Shohei Koide. Phage display for engineering and analyzing protein interaction interfaces. Current Opinion in Structural Biology 2007, 17:481–487.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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