Creative Biolabs, a world-leading service provider of antibody generation and development, has established a series of in vitro diagnostic (IVD) antibody development services for diagnostic uses, which are available for clients worldwide. Our scientific group focused on IVD antibody development is pleased to assist you in your projects with a timely and cost-effective way.
BRCA2 Marker
BRCA2 protein is encoded by the BRCA2 gene, which is a human tumor suppressor gene (to be specific, a caretaker gene). BRCA2 is alternatively called by the synonym breast cancer type 2 susceptibility protein and is responsible for repairing DNA. It is a 3418-amino acid protein, whose structure encompasses a single BRC repeat (BRC repeat 4; BRC4) crystallized with the RecA homology domain of Rad51. Just like BRCA1, BRCA2 is also commonly expressed in the cells of breast and other tissue, where it can contribute to the repair of damaged DNA. BRCA2 plays a vital role in the repair of DNA double-strand breaks (DSBs) by homologous recombination. BRCA2 is involved in a vital step of homologous recombination that it can bind the single strand DNA and directly interacts with the recombinase Rad51 to stimulate strand invasion. Moreover, BRCA2 protein has a certain function in maintaining the stability of the human genome and averting dangerous gene rearrangements that can result in hematologic and other cancers.
Fig.1 The locations of identified domains in human BRCA2 and the 27 exons that encode this protein.1
The Role of BRCA2 in Ovarian Cancer
It has been known that many cancers have epigenetic deficiencies in various DNA repair gene, and these repair deficiencies may cause increased unrepaired DNA damages. Studies demonstrate over-expressed BRCA2 in some types of cancers while other studies report under-expression of BRCA2. The over-expression of BRCA2 found in many cancers may indicate that compensatory BRCA2 over-expression and increased homologous recombinational repair can at least partially settle such excess DNA damages. Plenty studies have identified hundreds of mutations in the BRCA2 gene, many of which can raise the risks for cancer. Because of these mutations, the BRCA2 protein is abnormal and thus does not function properly. The defective BRCA2 protein is supposed to be unable to repair DNA damage that occurs throughout the genome. Consequently, there would be an increase in mutations due to error-prone translesion synthesis past un-repaired DNA damage, and some of these mutations can lead to tumorigenesis by causing cells to divide in an uncontrolled way and form a tumor.
Fig.2 Ovarian cancer susceptibility genes present in the double-strand DNA break repair pathway.2
Antibodies are core elements for antibody-based immunoassays for detecting and quantifying antigens of interest in all kinds of samples such as serum, urine, and tissue preparations. With advanced technology and years of experience, Creative Biolabs has launched a whole series of BRCA2-specific antibody development services for the diagnosis and prognosis of ovarian cancer. Additionally, Creative Biolabs also provides diagnostic immunoassay development services targeting this marker. We offer one-stop services from antibody generation to kit manufacture. If you are interested in our services, please feel free to contact us for more details.
References
For Research Use Only.
