Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of cholesterol. As an end-to-end company offering high-quality analytical development and qualification services, we have the best advantages in assisting our worldwide customers.

Introduction of Cholesterol

Cholesterol, with the molecular formula C₂₇H₄₆O, is a chemical organic compound that is generated during food processing. It's a key component of mammalian cells, with undoubtedly biological significance. Cholesterol may be found in large concentrations within the liver, spinal cord, and brain. Cholesterol is an important hydrophilic component of cell membranes, providing stability. It is a major precursor for the synthesis of vitamin D, bile acid, steroid hormones, and several toxic molecules with highly toxic and pathogenic activities.

Hypercholesterolemia, featuring an elevated level of cholesterol, especially the oxidized low-density lipoproteins (LDL), often promotes the formation of atheroma in the walls of arteries, a condition known as atherosclerosis, which may lead to coronary heart disease, strokes, and peripheral artery disease. High-density lipoprotein (HDL) extracts cholesterol from cells and atheromas. In contrast, hypocholesterolemia, featuring low cholesterol levels seems to be a consequence that may result from depression, cancer, and cerebral hemorrhage. Thus, the occurrence and distribution in the body fluid of different cholesterol types, HDL, LDL, and very-low-density lipoprotein (VLDL) have been widely used as important biochemical parameters for toxicity assessment, like atherosclerosis.

Fig.1 A fluorescent method for the detection of cholesterol. (Sun, et al., 2017)

Cholesterol Production Measurement Services at Creative Biolabs

The accumulation of cholesterol or its subfractions may exert toxic activities against healthy bodies. Therefore, it is imperative to monitor the body's level of cholesterol to bridge the quantitative efforts with a dependable risk assessment in the development process of candidate drugs or vaccines. Considering its importance in the evaluation of toxic effects, cholesterol measurement has been researched well by scientists of Creative Biolabs.

There are many methods and techniques have been proposed in analytical practice for cholesterol determination. Gas-liquid chromatography (GC) and GC/mass spectrometry (MS) have been successively used for the determination of total serum cholesterol. High-performance liquid chromatography (HPLC) and HPLC/MS have been used to determine total and free cholesterol. The enzymatic approaches involved with cholesterol oxidases or cholesterol esterases in the cholesterol biochemical reactions have been used to quantify cholesterol by measuring the consumption of substrates or the increment of production.

Quantitative determination of VLDL, LDL, HDL, and Lipoprotein(a) could be achieved by separation with agarose gel electrophoresis and density gradient ultracentrifugation/precipitation combined with the cholesterol subfractions measurement by enzymatic staining, densitometry, and near-infrared spectroscopy methods. A fluorescent method, shown in Fig.1, with excellent sensitivity and selectivity to cholesterol also could be used to detect cholesterol in practical serum samples These methods are accurate, specific, and capable of automation and the technique is generally adopted as a routine procedure for cholesterol measurement.

With decades of in-depth study of the toxicity assessment area, Creative Biolabs is one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies. With dedicated laboratory equipment and specially trained technicians, the detection procedures could be performed on semi-automatic or fully automated biochemistry analyzers, making it a routine service at Creative Biolabs.

Normally, cholesterol is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like total protein, electrolytes, alanine aminotransferases (ALT), and creatine kinase. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your fitness project. If you are interested in this, please feel free to contact us directly.

References

1. Richmond W. Analytical reviews in clinical biochemistry: the quantitative analysis of cholesterol. Annals of clinical biochemistry. 1992, 29(6): 577-597.
2. Sun, Q.; et al. Fluorometric detection of cholesterol based on β-cyclodextrin functionalized carbon quantum dots via competitive host-guest recognition. Talanta. 2017, 167: 513-519.

For Research Use Only | Not For Clinical Use