Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of glucose. As an end-to-end company offering high-quality analytical development and qualification services, we have the best advantages in assisting our worldwide customers.

Introduction of Glucose

Glucose is the most abundant and important monosaccharide as a source of energy with the molecular formula C₆H₁₂O₆. Insulin and glucagon are two vital hormones of the pancreas to keep glucose at stable levels in the blood. The hormone insulin promotes the cells of muscles, liver, and fat to uptake or store glucose from the blood for energy. The antagonistic hormone glucagon triggers the cells in the liver and muscles to release glucose into the blood. Intracellularly, the absorbed glucose enters the glycolysis process and gets broken down into pyruvate or lactate for energy generation.

The disorder of insulin leads to abnormally high levels of glucose, which will turn into type II diabetes over years. Diabetes damage the nerves and vessels, and increases the risk of stroke, heart attack, kidney disease, and vision loss. Higher concentrations of glucose may cause glycation of extracellular proteins diverted to the metabolic pathways shown in Fig.1, resulting in body toxicity, like neurotoxicity. Thus, glucose has been widely used as a biochemical parameter for toxicity assessment.

Fig.1 Physiological and pathophysiological metabolic mechanisms of glucose. (Tomlinson & Gardiner, 2008)

Glucose Production Measurement Services at Creative Biolabs

Considering the importance of glucose toxicity research in pharmacological development, glucose measurement has been researched well by scientists of Creative Biolabs. There are many methods and techniques have been proposed in analytical practice for glucose determination. The physiological fluid samples, such as blood, serum, plasma, interstitial fluid, saliva, and urine, could be collected by invasive or noninvasive methods. Devices to measure glucose could be physically categorized into electrochemical devices, piezoelectric devices, thermoelectric devices, acoustic devices, and optical devices, among which the electrochemical method has the advantage of low cost, portability, and high sensitivity. For the device construction, electrodes of enzymes, like enzyme glucose oxidase (GOD), are incorporated. Glucose could be determined by the measurement of the oxidation or reduction of substances or by the detection of the changes in pH or electrical resistance during these reactions catalyzed by specific enzymes.

With decades of in-depth study of the toxicity assessment area, Creative Biolabs is one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies. With dedicated laboratory equipment and specially trained technicians, the detection procedures could be performed on semi-automatic or fully automated biochemistry analyzers, making it a routine service at Creative Biolabs.

Normally, Glucose is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like total protein, electrolytes, alanine aminotransferases (ALT), and creatine kinase. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your project. If you are interested in this, please feel free to contact us directly.

References

1. Song, L. Glucose and bone metabolism indices. Advances in clinical chemistry. 2017, 82: 1-46.
2. Goldstein, D.A. Serum Glucose. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. 1990.
3. Tomlinson, D.R.; Gardiner, N.J. Glucose neurotoxicity. Nature Reviews Neuroscience. 2008, 9(1): 36-45.

For Research Use Only | Not For Clinical Use