Introduction to Anti-Cell-Based Target Antibody Discovery by Premade Antibody Library

With the deepening of antibody research, scientists have discovered more and more types of antibodies, such as functional human antibodies, anti-cell-based target antibodies,bifunctional antibodies, and anti-epitope antibodies. Meanwhile, based on different types of antibodies, corresponding methods of antibody development have been developed. The anti-cell-based target antibody is a type of antibody that targets the whole cell. It recognizes and binds to multiple molecules on the cell surface. These antibodies can act on cells in a variety of ways, such as inducing apoptosis, activating the immune system, and preventing cell growth and spreading. In clinical practice, antibodies against whole cell antigens have been widely used to treat a variety of malignant tumors, such as leukemia, lymphoma, and melanoma. Based on our rich field experience and advanced research platform, Creative Biolabs provides comprehensive services to support premade antibody library screening and anti-cell-based target antibody discovery.

Anti-Cell-Based Target Antibody

When we are not sure of the antigen we need, such as when we want to prepare antibodies to tumor-specific antigens, we are unable to find suitable peptides and proteins to use as antigens. Consequently, the commonly used methods for the preparation of anti-peptide/protein antibodies would not be applicable in this case.

In this case, it is often necessary to immunize tissue, whole cells, or cell fractions as antigens to prepare antibodies. If whole cells are used as the immunogen, the serum in the culture medium needs to be washed away. It is recommended to use intact cells directly as the immunogen without breaking them up, because intact cells have stronger granularity and exogenous origin. Tissue-derived cells and artificially cultured cells are preferred for the immunization of animals by the intraperitoneal route rather than subcutaneous immunization.

In addition, antibody preparation based on whole-cell antigen presentation is an important mode. In the initiation step of immunization in mammals, antigens are not directly processed by Th cells, but antigen-presenting cells do the processing and presentation of antigens. If we can prepare cells that express the target antigens, then these antigens will be expressed in large quantities on the surface of this cell, similar to antigen-presenting cells, so that their surface carries antigenic determinants. This cell can be then used to immunize the animal with antigens. This is the eukaryotic system's in situ expression of transmembrane proteins, which can ensure the complete post-translational modification processing of antibodies. At the same time, the natural structural features are well preserved due to the use of the cell membrane as the assembly display surface.

Antibodies prepared using whole cells as antigens also have disadvantages. Because the cell volume is too large compared to the target antigen and the surface has certain modifications of the non-target antigen, its use as an antigen, although the immunogenicity can be guaranteed the effectiveness and specificity are difficult to ensure, the solution to this problem includes the high efficiency of the expression of the target antigen as well as a large number of later screenings that are required to confirm and exclude the recognition of ineffective antigen antibodies.

Creative Biolabs has a wealth of knowledge and experience in the premade antibody library screening. We would be happy to share with you our knowledge and experience in premade phage displayed human antibody library construction and anti-cell-based target antibody discovery.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: