Introduction to Thermal Stable mAbs Discovery by Phage Display

Monoclonal antibodies possess remarkable targeting properties and exhibit exceptionally high specificity, making them a topic of widespread interest. With the increasing application of antibodies in biomedical research and other fields, the demand for antibodies continues to rise. However, being biological macromolecules, antibodies are susceptible to various factors, such as temperature and pH, which can significantly affect their activity. Therefore, enhancing the stability of monoclonal antibodies is a critical issue that must be addressed for their widespread application. One commonly employed solution is the screening of thermally stable monoclonal antibodies using phage display technology. Specific screening procedures designed based on experimental requirements, leading to the identification of ideal thermally stable monoclonal antibodies. Leveraging our extensive expertise and advanced phage display platform, Creative Biolabs offers comprehensive services to support the development of thermally stable monoclonal antibodies.

Antibodies (Creative Biolabs Authorized)

Thermal Stable Monoclonal Antibody

Temperature exerts varying degrees of influence on the structure and activity of monoclonal antibodies. High temperatures can disrupt antibody structures, often irreversibly, leading to aggregation. Therefore, the screening of thermally stable monoclonal antibodies holds paramount importance for both research and applications.

Two essential types of monoclonal antibodies are single-chain antibodies (scFv) and antigen-binding fragments (Fab). In comparison to classical antibody molecules, scFv is characterized by its small molecular weight (approximately 1/6th that of a complete antibody molecule). It was initially successfully expressed in yeast and plant cells, where it can spontaneously fold into a natural structure with antigen-binding properties. scFv finds diverse applications in antiviral therapies, tumor treatments, autoimmune disease therapies, targeted drug therapies, etc. On the other hand, Fab comprises both antigen-binding and partially constant regions, rendering it invaluable for research and application. Fab's dual regions grant it similar antibody-antigen affinity and excellent tissue penetration capabilities, alongside a more stable structure. This stability is crucial for its role in clinical diagnosis and treatment. However, the impact of high temperatures on scFv and Fab limits their extensive application. Therefore, the screening of thermal stable scFv/Fab is vital for expanding their applications.

Additionally, VHH lacks the light chain, and its heavy chain variable region's CDR3 region is lengthy, making it the smallest known unit capable of binding antigens. This characteristic imparts several advantages, including low immunogenicity, high solubility, strong tolerance, and excellent tissue permeability. VHH finds widespread use in disease diagnosis and treatment. Nevertheless, temperature acts as a barrier, hindering the broader application of VHH. Therefore, screening of thermal stable VHH is crucial to broaden its application scope. Furthermore, studies have indicated that VHH itself exhibits higher temperature resistance compared to classical antibodies, facilitating the screening process for thermal stable VHH.

Phages represent stable macromolecules, providing a robust foundation for phage display technology, which opens up new avenues for screening thermally stable monoclonal antibodies. Through the construction of phage display variant libraries, multiple rounds of monoclonal antibody screening can be conducted. Tailored temperature gradients are set for screening with each round involving isolation, propagation, and enrichment. These steps are essential for identifying monoclonal antibody variants that maintain their folding and activity.

More Details on Thermal Stable mAbs Discovery by Phage Display

Introduction to Thermal Stable scFv/Fab Screening by Phage Display
Introduction to Thermal Stable VHH Screening by Phage Display

Creative Biolabs possesses extensive knowledge and experience in the screening of thermally stable monoclonal antibodies. We are eager to share our expertise with you.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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