Scaffold Library Introduction

Antibody display technology is a technology that uses the surface of a vector such as bacteria, yeast, or phage to display antibodies or their fragments. In contrast, engineered protein scaffolds are transverse, blocky protein structures with favorable biophysical and chemical properties that can be engineered to have specific functions or specificities for binding to diverse targets. These scaffolds can be used in the fields of drug delivery, tumor therapy, immunotherapy, tissue engineering, and biosensors. When screening high affinity binding proteins, engineering scaffolds can be used as carriers of the replacement antibody. Based on our rich practical experience and advanced technology platform, Creative Biolabs provides sufficient services to support phage display library construction, including immune antibody libraries, scaffold libraries, peptide libraries, and cDNA library construction.

Engineered Scaffold Protein

Engineered protein scaffolds are invaluable biotechnology and pharmaceutical application tools due to their modular structure and favorable biophysical and chemical properties such as small size, high cell membrane permeability, no disulfide bonds within the molecule, high thermodynamic stability, and no aggregation. These properties not only allow for specific and high-affinity binding to a diverse range of targets but also suggest the use of rigid protein frameworks for other areas, such as universal engineering concepts, intracellular applications, and others. Currently, the application of engineered protein scaffolds has expanded to include X-ray structure determination of complex/target complexes, imaging of cell surface markers, and conjugation with drug payloads. Of course, these are also categories that have entered the clinical trial phase. The constructed engineered protein scaffold library can be used as an option for antibody carrier screening of high affinity binding molecules.

Trimer Codon Technology

Trimer codon technology is a method used for oligonucleotide-directed mutagenesis. It is a unique line of products known as trimer phosphoramidites that allow customers to effectively synthesize oligonucleotides based on amino acid codons rather than individual nucleotides. The use of 2'-deoxynucleoside trimeric phosphoramidite is a particularly effective way to deal with oligonucleotide-directed mutagenesis. In 64 possible combination codes, you just need 20 codons covering 20 kinds of amino acids. Unlike other mutagenesis methods, libraries constructed from trimeric oligonucleotides are free of codon bias, no stop codon generation, and custom mixtures allow control of codon abundance, making it one of the most effective tools for exploring functionally important sequence spaces in protein regions—even under unsaturated conditions. In chemistry, a trimer is a molecule or polyatomic anion formed by the combination or association of three molecules or ions of the same substance.

Engineered protein display scaffolds that have been specifically selected can bind to almost any target. Protein engineering was a prerequisite for the emergence of all protein scaffolds and remains the driving force behind this process. And scaffold libraries can greatly expand the range of applications for specific protein scaffolds. Trimeric codon technology-based mutant library construction techniques allow for absolute precision in mutant library construction and can provide scaffold libraries in excess of 10¹⁰ sizes.

Creative Biolabs has a wealth of knowledge and experience in phage display library construction. We would be happy to discuss with you our knowledge and experience in scaffold libraries.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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