Chromatin Immunoprecipitation (ChIP) Sequencing Service
Background Services Advantages Q&As Resource
As an experienced contract research organization (CRO), Creative Biolabs is dedicated to providing comprehensive epigenomics services to facilitate your cancer epigenomic research using the world renowned expertise in oncology, epigenomics, next-generation sequencing(NGS) etc. We strive to provide our customers with superior quality data to ensure rapid and successful progression of your programs.
Background
Chromatin consists of DNA, histones and non-histone proteins. DNA, tightly wrapped around histones, is critical for the accessibility to DNA binding proteins, such as transcription factors, leading to the regulation of specific gene expression. To investigate more details about the regulation of gene expression, it’s necessary to explore the epigenetic roles of histones and transcription factors. Chromatin immunoprecipitation sequencing (ChIP-seq) is one of the epigenetic approaches to map DNA-binding proteins and histone modification on a genome-wide scale. Sequencing the target regions of DNA binding proteins, exploring the DNA-protein interaction, profiling histone modification are crucial for understanding cancer-associated cellular processes.
Fig.1 Overview of a ChIP-seq experiment. (Park, 2009)
Our Service
To profile the DNA/protein interaction and map genome-wide histone modification in cancer research, Creative Biolabs has established a superior ChIP-seq platform to advance and maximize the success of your epigenetic research. Compared with traditional array-based ChIP, ChIP-seq can offer higher resolution and more accurate data. Our end-to-end ChIP-seq services include chromatin sample preparation, ChIP-seq library construction, Next-generation Sequencing and data analysis (see Fig.2). We provide 100% guarantee for the whole package service.
Fig.2 Workflow of ChIP-seq service.
Advantages
We care for your samples. Our highly experienced scientists and technicians are committed to following the most strict quality procedures to ensure the accurate data output and analysis. As a powerful technology, ChIP-seq can generate millions of counts for various samples in a cost-effective, precise and unbiased manner. In addition, our advantages include:
- Highly efficient enrichment of targeted DNA;
- Increase sensitivity, specificity and flexibility;
- Fixed cell pellets or frozen tissues are both feasible;
- Capture DNA targets across the whole genome.
Fig.3 Examples of the profiles generated by ChIP-seq and ChIP-chip. (Park, 2009)
As a flexible partner in the cancer epigenetic research, Creative Biolabs aspires to become the most trusted solution provider for ChIP-seq. We are dedicated to increasing the probability of success and reducing the overall program timeline for our clients. Please don’t hesitate to contact us to know more.
Q&As
Q: What sample requirements are needed for ChIP-Seq?
A: Typical sample requirements for ChIP-Seq include enriched DNA samples with a concentration of at least 2 ng/μL and fragment sizes between 100 bp and 500 bp. High-quality DNA with minimal contamination is essential to ensure reliable results.
Q: How is ChIP-Seq data analyzed?
A: ChIP-Seq data analysis involves several steps: data quality control, mapping reads to a reference genome, peak calling to identify protein-binding sites, motif prediction, and functional annotation of peaks. Bioinformatics tools like MACS2 are often used for peak calling and downstream analyses.
Q: What are the data output formats for ChIP-Seq?
A: The data output from ChIP-Seq typically includes raw sequencing reads in FASTQ format, aligned reads in BAM format, and peak call files in BED format. Additional files for differential peak analysis, motif analysis, and functional annotation may also be provided, depending on the bioinformatics pipeline used.
Q: Can ChIP-Seq be integrated with other genomic techniques?
A: Yes, ChIP-Seq can be integrated with other genomic techniques such as RNA-Seq, ATAC-Seq, and whole-genome bisulfite sequencing. Combining these methods provides a comprehensive view of gene regulation, chromatin accessibility, and DNA methylation patterns, offering deeper insights into the epigenetic landscape of cancer.
Q: What are the key applications of ChIP-Seq in cancer research?
A: ChIP-Seq is used to map histone modifications, identify binding sites of transcription factors, and study chromatin structure changes. These applications help in understanding epigenetic changes, gene expression regulation, and the identification of novel therapeutic targets in cancer cells.
Reference
- Park, Peter J. "ChIP-seq: advantages and challenges of a maturing technology." Nature Reviews Genetics 10.10 (2009): 669.
Resources
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Note: Our sequencing services are for Research Use Only. Not For Clinical Diagnosis.
Related Services:
- Whole Genome Bisulfite Sequencing (WGBS) Service
- Reduced-representation Bisulfite Sequencing (RRBS) Service
- Targeted Methylation Sequencing Service
- Chromatin Accessibility Sequencing Service
- Epigenomic Sequencing Service for Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
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