Anti-Membrane Protein Antibody Production by DNA Immunization

As a world-leading service provider in the field of anti-membrane protein antibody discovery, Creative Biolabs is dedicated in leveraging our experience and technologies to assist with custom projects. Here, Creative Biolabs is proud to introduce our well-established DNA immunization service for custom anti-membrane antibody development. As an advanced strategy, DNA immunization enables in vivo expression of the membrane protein of interest in immunized hosts, thus raising potent immune responses without the need for immunogen protein preparation.

Membrane proteins, such as G-protein coupled receptors (GPCRs) and ion channels, are attractive drug targets. Therefore, anti-membrane antibodies have been widely applied for treatment of various cancers, autoimmune diseases, infectious diseases, etc. However, in terms of membrane proteins that are general large molecules with multiple domains and complicated structures, traditional protein-based immunization approaches sometimes meet difficulties in immunogen preparation: how to produce the protein with intact structure and function activities, meanwhile suitable for animal immunization? DNA immunization provides an innovative solution to this issue. This straight-forward method can bypass the time- and labor-consuming immunogen production phase through directly introducing DNA encoding target protein into host cells.

Introduction to DNA immunization

DNA immunization is one of the most straight-forward approaches to elicit an immune reaction against a foreign protein. In contrast to traditional protein immunization, this method allows in vivo antigen production, circumventing immunogen preparation stage, which, especially for membrane proteins, can be very difficult. Meanwhile, as is known, the structural integrity of proteins is critical for the induction of functional antibodies. While, the sensitive structures of membrane proteins tend to be lost during the in vitro production process, regardless of whether they are produced as recombinant proteins or are extracted directly from natural sources. Therefore, expressing intact immunogens in vivo by DNA immunization can maximally restore the native status of the target protein (e.g. PTMs, structure), enabling higher chances of raising anti-membrane antibodies with the desired biological activities, which is crucial for therapeutic antibody development.

Fig 1 Example of DNA used as immunogen in hybridoma approach.

DNA immunization can be well incorporated with hybridoma technology, phage display immune library technology or Native™ antibody discovery for antibody development against membrane proteins. It’s proved to be a powerful approach to produce high-quality anti-membrane antibodies and offers several unique advantages:

Highly efficient for testing different immunogen designs
No requirement for production/purification of membrane proteins, avoiding the time- and effort-consuming process
Maximally restoring native status of target proteins
Minimal cytotoxicity to the hosts
Robust and potent immune responses
Suitable for generating antibodies against conformation-sensitive membrane protein targets
A wide range of hosts available, such as mouse, rabbit, camel etc.

With extensive experience in hundreds of successful cases in this field, scientists in Creative Biolabs has earned great reputation from our worldwide customers for whom we have developed ideal anti-membrane protein antibodies. Our skillful team has rich experience in dealing all kinds of membrane proteins, such as GPCR immunogens, ion channel immunogens, and transporter immunogens. We are confident to tailor first-class, custom-oriented services to address every specific requirement. Please feel free to contact us for more information.

Reference

Liu S, Wang S and Lu S. (2016). DNA immunization as a technology platform for monoclonal antibody induction. Emerging Microbes and Infections. 5, e33.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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