Antibody Library for In Vitro Display

In vitro display technologies (phage display, bacterial display, yeast display, ribosome display, mRNA display) provide powerful ways for isolating high-affinity antibodies from small, focused libraries. Creative Biolabs has extensive experience in building in vitro display antibody libraries in varied formats and display types. As long as you provide the antigen for us, we can provide immunization, design, and construction services of the antibody libraries for you.

The diverse repertoire of antibody molecules is a result of the combination of six complementary determining regions (CDRs), three from the heavy (H) and three from the light (L) chain variable domains (V_H, V_L), and contained within V domain frameworks. In B cells, the complete V regions are obtained by DNA rearrangement. After cell activation, different gene segments are combinatorial assembled by somatic mutation. Display technologies use recombinant DNA methods to generate combinatorial DNA libraries for selection. By recombinant DNA methods, the diversity and creation of novel sequences are expanded which are not available to the immune system in vivo.

Creative Biolabs provides several types antibody libraries for the selection and screening of high-affinity antibodies. With large library construction, we have the ability to find specific, high-affinity binders to particular epitopes. The types of combinatorial antibody libraries including:

Immune antibody library
Naïve antibody library
Semi-synthetic antibody library
Synthetic antibody library

All of these library types have been proven to be useful sources for the selection of antibodies for research, diagnostics, and therapy.

Antibody Libraries for In Vitro Display

Advantages of Combinatorial Antibody Libraries

Nowadays, there are many different methods to construct large antibody libraries by genetic engineering. Basically, the construction of antibody libraries involves constructing a large number of different light chains and heavy chains into vectors and using an expression system links genotype (the antibody gene) and phenotype (the antibody molecule).

There are many advantages of combinatorial antibody libraries

More acceptable and cheaper. They allow the creation of a synthetic immune system in a test tube, other than relying on live animals for generating antibodies.
Circumvent immune tolerance. Most therapeutic antibodies are designed to recognize self-antigens.
Can generate human-derived antibodies.
Have the ability to generate rare antibodies.
Large library size (approximately 10¹¹-10¹² members).
Allow understanding several characteristics of antibody and improving the characteristics.
Higher affinity compared with naturally occurring antibodies (in the pM versus nM range).
May lead to new innovative uses for antibodies.

For more detailed information, please feel free to contact us or directly sent us an inquiry.

References

Hust M, Dübel S, and Schirrmann T (2007) “Selection of recombinant antibodies from antibody gene libraries.” Methods Mol Biol 408:243-255. doi: 10.1007/978-1-59745-547-3_14.
Barbas C F, Bain J D, Hoekstra D M, et al. (1992) “Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.” Proc Natl Acad Sci U S A 89(10):4457-4461.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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