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pCDCAR1 5T4 h(28ζ) (CAR-ZP086)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-5T4 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human 5T4. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-5T4 antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of acute lymphoblastic leukemia (ALL),acute myeloid leukemia (AML),chronic lymphocytic leukemia (CLL),chronic myeloid leukemia (CML).

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Details

  • Target
  • 5T4
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Acute lymphoblastic leukemia (ALL)，Acute myeloid leukemia (AML)，Chronic lymphocytic leukemia (CLL)，Chronic myeloid leukemia (CML)
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-CD3ζ
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR T cell containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • H8
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • TPBG
  • Synonyms
  • 5T4, 5T4AG, M6P1, WAIF1

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  • Published Data
Complete CAR data FuncS

Fig.1 Percentage of EpCAM+ 5T4+ cells in the tumor disaggregates.

CAR Construction : H8 scFv-41BB-CD3ζ Latest CAR Construction

Fig.1 Percentage of EpCAM+ 5T4+ cells in the tumor disaggregates.

5T4 expression on FFPE sections of tumor biopsies from patients with ovarian cancer.

Owens, G. L., Sheard, V. E., Kalaitsidou, M., Blount, D., Lad, Y., Cheadle, E. J., Edmondson, R. J., Kooner, G., Gilham, D. E., & Harrop, R. (2018). Preclinical Assessment of CAR T-Cell Therapy Targeting the Tumor Antigen 5T4 in Ovarian Cancer. Journal of immunotherapy (Hagerstown, Md. : 1997), 41(3), 130–140.
Complete CAR data FuncS

Fig.2 Kaplan-Meier survival curves of NSG mice receiving ascending doses of H8-CAR T cells.

CAR Construction : H8 scFv-41BB-CD3ζ Latest CAR Construction

Fig.2 Kaplan-Meier survival curves of NSG mice receiving ascending doses of H8-CAR T cells.

NSG mice were challenged with 2.5×106 SKOV-3 tumor cells on day 0 and 7 days later were treated with either ascending doses of H8-CAR T cells or saline.

Owens, G. L., Sheard, V. E., Kalaitsidou, M., Blount, D., Lad, Y., Cheadle, E. J., Edmondson, R. J., Kooner, G., Gilham, D. E., & Harrop, R. (2018). Preclinical Assessment of CAR T-Cell Therapy Targeting the Tumor Antigen 5T4 in Ovarian Cancer. Journal of immunotherapy (Hagerstown, Md. : 1997), 41(3), 130–140.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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