• One-Stop CAR-T Therapy Development
  • Biomarker Identification & Selection
  • ScFv Generation
  • CAR Design & Construction
  • CAR-T Gene Packaging & Delivery
  • Replication-Competent Virus Testing
  • CAR Cell In Vitro Assay
  • CAR-T Preclinical In Vivo Assay
  • IND Development for CAR-T Cell Therapy
  • GMP Production for CAR-T Products
  • CAR Clinical Trial
  • One-Stop Canine CAR-T Therapy Development
  • One-Stop mRNA CAR-T Therapy Development
  • TRAC-CAR-T Cell Development
  • Modular CAR-T Cell Generation
  • One-Stop Silence™ CAR-T Cell Development
  • Vaccine Boosting CAR-T Cell Therapy
  • STAb-T Cell Therapy Development
  • CAR-T Therapy Development in the Non-Cancer Field
  • Anti-HIV CAR Cell Therapy Development
  • T Cell Activity Modulator Discovery
  • T Cell Programming
  • Virus Specific T Cell (VST) Development
  • Antibody-Coupled T Cell Receptor (ACTR) Development
  • Organoid-induced hPSC-original T Cell Differentiation
  • γδ T Cell Engineering
  • CAR-T CDMO
• One-Stop TCR-T Therapy Development
  • TCR Biomarker Identification & Selection
  • TCR Generation & Optimization
  • TCR Design & Construction
  • TCR Gene Packaging & Delivery
  • TCR In Vitro Assay
  • TCR-T Preclinical In Vivo Assay
  • TCR Clinical Application
  • TCR Analysis
  • TCR Tetramerization Construction
  • ScFv-TCR T Cell Therapy Development
  • AbTCR Development
  • Bispecific TCR Development
  • CER Development
• One-Stop CAR-MA Therapy Development
• One-Stop CAR-NK Therapy Development
  • One-Stop CAR-NKT Therapy Development
  • One-Stop UniCAR-NK Therapy Development
  • iPSC-derived CAR-NK Therapy Development
  • One-Stop TCR-NK Therapy Development
  • NK Cell Genetic Engineering
  • NK Cell Expansion
• One-Stop CAR-B Therapy Development
• One-Stop CAR-Monocyte Therapy Development
• One-Stop Cytokine Release Syndrome (CRS) Management
• TCR-Like Antibody Development
  • MHC/Peptide Complex Production
  • TCR-Like Antibody Discovery
  • TCR-Like Antibody Affinity Characterization
  • TCR-Like CAR Construction & Evaluation
• In Vivo Cell Engineering Development
  • In Vivo CAR-T Cell Engineering
  • In Vivo B Cell Engineering
  • In Vivo CAR-NK Cell Engineering
  • In Vivo CAR-M Cell Engineering
• One-Stop Allogeneic Cell Therapy Development
  • Allogeneic CAR-T Therapy Development
  • Allogeneic TCR-T Therapy Development
  • Allogeneic CAR-NK Therapy Development
  • Allogeneic CAR/TCR Cell Design & Engineering
  • Other Allogeneic Cell Therapy Development
• TCR/CAR/Antigen-Engineered Tregs Development
  • CAR-Treg Development
• Cancer Vaccine Development
  • Cancer Vaccine Discovery
  • Cancer Vaccine Engineering
  • Cancer Vaccine Analytical Characterization
  • Cancer Vaccine In Vivo Assessment
  • Cancer Vaccine GMP Manufacturing
  • Dendritic Cell Vaccine Development
• Immune Genotyping
  • HLA Typing
  • KIR Typing
  • HLA Testing
  • Dog DLA Genotyping
  • Guinea Pig GPLA Genotyping
  • Mouse H2 Genotyping
  • Rabbit RLA Genotyping
  • Rat RT1 Genotyping
  • Rhesus Macaque MHC Genotyping
• Other Services
  • Tumor-infiltrating Lymphocyte-based Antibody Discovery

TCR Tetramerization Construction Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

TCR Tetramer

Peptide-MHC tetramers have been extensively applied for the detection and quantification of antigen-specific T cell populations and research MHC functions. Based on a powerful TCR development platform, scientists at Creative Biolabs have experience in the generation and optimization of TCR. We extend tetramerization technology to TCRs and have the ability to manufacture custom TCR tetramers that bind MHC-peptide complexes with high affinity.

Fig.1 TCR tetramer.

TCR tetramers serve as surface staining reagents for analysis of the MHC-peptide interface and the design of immunotherapeutics and vaccines. Compared to TCR monomers, TCR tetramers have higher affinities and can extend the half-life of TCR:pMHC interactions. TCR tetramers can provide new insight into MHC–mediated antigen presentation.

Fig.2 TCR monomer vs. TCR tetramer.

Potential Applications of TCR Tetramer

• Detection of target cells pulsed with cognate peptide
• Quantification of antigen changes at the cell surface
• Identification of cross-reactive peptides
• Study of interactions between T cells and APCs
• Identification of virus-infected cells
• Competitive inhibition of antigen-specific CTL activation
• Antigen-directed drug delivery

Preparation of TCR Tetramers

At Creative Biolabs, our team of scientists is dedicated to providing customized TCR tetramers to meet the needs of customers. Provide us with your candidate's TCR sequences or your specific TCR of interest, we can custom manufacture a TCR tetramer based on your needs.

A TCR Tetramer is prepared using biotinylated specific TCRs and fluorophore conjugated streptavidin which has four biotin binding sites.

• Generation of Soluble TCR Chains
  Soluble TCRs have been expressed in various recombinant protein expression systems. For example, insect cells and bacterial expression systems (E. coli) are widely used to support the expression of the gene of interest. Two polypeptide chains (α and β chains ) of TCR are expressed separately from two distinct vectors and paired by a non-native disulfide bond. A biotinylation target motif is also fused to the C terminus of the TCR β chain to allow tetramerization with extra-virgin.
• Refolding Soluble Monomeric TCRs
  Purified TCR chains are refolded for several days to obtain soluble TCR monomers.
• Biotinylation of Monomeric TCRs
  The refolded monomer is labeled with biotin.
• Multimerization of Refolded TCRs
  TCR monomers are tetramerized by the addition of fluorochrome-conjugated streptavidin to the monomers.

Service Request

Please contact us about your custom TCR tetramer request.

Reference

1. Low, J.L.; et al. Binding of TCR multimers and a TCR-like antibody with distinct fine-specificities is dependent on the surface density of HLA complexes. PloS one. 2012, 7(12): e51397.