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Anti-ANTXR1 KIR CAR (XW-14) Vector (XS-1122-YF14)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-ANTXR1 KIR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human ANTXR1. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv (XW-14) of anti-ANTXR1 antibody linked to KIR2DS2 TM & ICD and DAP12. The vector product was designed for the discovery and development of cellular therapy against Gastric, breast, colon, and pancreatic tumors. This KIR signaling is designed to prolong KIR-CAR T cells' efficacy period by coupling the receptor chains during activation and decoupling them during rest, avoiding T cell exhaustion even in challenging solid-tumor environments.

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Details

  • Target
  • ANTXR1
  • Targeting Cell Type
  • T Cell
  • Targeting Diseases
  • Gastric, breast, colon, and pancreatic tumors
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-KIR2DS2 TM&ICD-2A-DAP12
  • Discription of Signaling Cassetes
  • The dual binding and signal chains are designed to come together to form a single receptor complex when bound to a tumor target. Dual chain signaling provides prolonged T cell activation even in challenging solid-tumor environments. KIR CAR combines the power of NK cell KIR receptors with the long-term durability of T cells, resulting in a long-lasting and powerful anti-cancer efficacy. KIR CAR-T cells maintained activation and potent cytotoxic activities in solid tumors compared with the CD28 and 4-1BB co-stimulated CD3-ζ -based traditional CAR T cells. The DAP12 signal functions as both activation and co-stimulation for T cells, circumventing the CD3-ζ pathway to allow prolonged KIR-CAR function.

Target

  • Clone
  • XW-14
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • ANTXR cell adhesion molecule 1
  • Synonyms
  • ATR; GAPO; TEM8
  • Introduction
  • This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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