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Anti-EphA2 (3F2)-Megf10 CAR-MA (CARMA-W0658)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-EphA2 chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human EphA2 and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-EphA2 antibody (3F2) linked to Megf10 signaling domains. The vector product was designed for the treatment of GBM, glioma.

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Details

  • Target
  • EphA2
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • GBM, glioma
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • 3F2
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • Megf10
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(Megf10)
  • Discription of Signaling Cassetes
  • Megf10:
    Megf10 is used as the CAR intracellular signaling domain. As a phagocytosis receptor, Megf10 has cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) that are phosphorylated by Src family kinases. Based on this mechanism, Megf10 signaling can induce macrophage phagocytosis which has been demonstrated in the published article.
    It is a membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. It's a receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells

Target

  • Target
  • EphA2
  • Target Species
  • Human
  • Clone
  • 3F2
  • Host
  • Human
  • Gene Name
  • EPH receptor A2
  • Introduction
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
  • Alternative Names
  • ECK; CTPA; ARCC2; CTPP1; CTRCT6; EPHA2; EPH receptor A2; Erythropoetin producing hepatocellular carcinoma A2

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Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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