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Anti-GPRC5D (X10X282) h(BBζ) pB CAR system (XS-1022-LX1104)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative biolabs provides highly effective CAR piggyBac vector system: Anti-GPRC5D (X10X282) h(BBζ) pB CAR system. Which contains two vectors: one vector, referred as the helper PBase plasmid, encodes the transposase. The other vector, referred to as the transposon plasmid, which is constructed for the engineering of T cells to target human GPRC5D. The T cells are genetically modified through transduction with the PB vector expressing scFv of anti-GPRC5D antibody linked to a 4-1BB, CD3-zeta signaling domains. And the vector product was designed for the treatment of lymphoma.

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Details

  • Target
  • GPRC5D
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • lymphoma
  • Generation
  • Second
  • Vector Name
  • piggyBac vector system
  • Vector Length
  • ~8kb
  • Vector Type
  • piggyBac transposon
  • Receptor Construction
  • scFv-41BB-CD3ζ
  • Discription of Signaling Cassetes
  • 41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigennonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric 4-1BB and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • X10X282
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • G protein-coupled receptor class C group 5 member D
  • Synonyms
  • GPRC5D; G protein-coupled receptor; class C; group 5; member D; G protein-coupled receptor class C group 5 member D

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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